Detailed information of TA system TA199166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1568322..1568949
• Replicon: chromosome
• Accession: NZ_CP072904
• Organism: Bartonella henselae strain Marseille/ URLLY-8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: X5MEM9
• Locus tag: KAE73_RS06740
• Protein ID: WP_038487056.1
• Coordinates: 1568322..1568636 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: KAE73_RS06745
• Protein ID: WP_034454130.1
• Coordinates: 1568653..1568949 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KAE73_RS06720 (KAE73_06600)	1563950..1565494	-	1545	WP_236095364.1	phage portal protein	-
KAE73_RS06725 (KAE73_06605)	1565494..1565742	-	249	WP_038487052.1	phage related protein	-
KAE73_RS06730 (KAE73_06610)	1565746..1567677	-	1932	WP_082251725.1	phage terminase large subunit family protein	-
KAE73_RS06735 (KAE73_06615)	1567708..1568259	-	552	WP_034454327.1	hypothetical protein	-
KAE73_RS06740 (KAE73_06620)	1568322..1568636	+	315	WP_038487056.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KAE73_RS06745 (KAE73_06625)	1568653..1568949	+	297	WP_034454130.1	HigA family addiction module antitoxin	Antitoxin
KAE73_RS06750 (KAE73_06630)	1569358..1569621	+	264	WP_038487057.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
KAE73_RS06755 (KAE73_06635)	1569608..1569889	+	282	WP_011180281.1	type II toxin-antitoxin system YafQ family toxin	-
KAE73_RS06760 (KAE73_06640)	1569932..1570210	-	279	WP_038487059.1	hypothetical protein	-
KAE73_RS06765 (KAE73_06645)	1570207..1570965	-	759	WP_038487061.1	antA/AntB antirepressor family protein	-
KAE73_RS06770 (KAE73_06650)	1570997..1571542	-	546	WP_236095365.1	antA/AntB antirepressor family protein	-
KAE73_RS06775 (KAE73_06655)	1571841..1572386	-	546	Protein_1324	antA/AntB antirepressor family protein	-
KAE73_RS06780 (KAE73_06660)	1572961..1573551	-	591	WP_038487063.1	antA/AntB antirepressor family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1522448..1593646	71198

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (12-104)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-80)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12135.97 Da
• Isoelectric Point: 9.0878

>T199166 WP_038487056.1 NZ_CP072904:1568322-1568636 [Bartonella henselae]
MIHKRTNEGDLVIESFADKRCKDLLEGNPPKGFPTTLVRIVQRKLFMLDKAVDLKDLRSPPGNRLDALKGERKGQYSIRI
NDQFRICFEWRSNGAYEVEIIDYH

Nucleotide

• Length: 315 bp

>T199166 NZ_CP097223:2331911-2332129 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11265.91 Da
• Isoelectric Point: 5.1546

>AT199166 WP_034454130.1 NZ_CP072904:1568653-1568949 [Bartonella henselae]
MKNYIAIHPGEILREEYLKEYALSAYALAKALNVPRTRIERIVAENSPVTPDTALRLAYFFDTTAEFWLNMQAAYDVSVL
QAEKADEFSKISKFECRV

Nucleotide

• Length: 297 bp

>AT199166 NZ_CP097223:2331510-2331884 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: X5MEM9