Detailed information of TA system TA199129

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 716036..716663
• Replicon: chromosome
• Accession: NZ_CP072900
• Organism: Bartonella henselae strain G-5436

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0H3M2H0
• Locus tag: KAE71_RS03280
• Protein ID: WP_011180277.1
• Coordinates: 716349..716663 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: KAE71_RS03275
• Protein ID: WP_011180278.1
• Coordinates: 716036..716332 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KAE71_RS03240 (KAE71_03225)	712014..712556	+	543	WP_011180285.1	phage antirepressor Ant	-
KAE71_RS03245 (KAE71_03230)	712588..713427	+	840	WP_011180284.1	antA/AntB antirepressor family protein	-
KAE71_RS03250 (KAE71_03235)	713444..713959	+	516	WP_011180283.1	phage antirepressor Ant	-
KAE71_RS03255 (KAE71_03240)	714019..714777	+	759	WP_011180282.1	phage antirepressor Ant	-
KAE71_RS03260 (KAE71_03245)	714774..715052	+	279	WP_011180242.1	hypothetical protein	-
KAE71_RS03265 (KAE71_03250)	715095..715376	-	282	WP_011180281.1	type II toxin-antitoxin system YafQ family toxin	-
KAE71_RS03270 (KAE71_03255)	715363..715626	-	264	WP_011180280.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
KAE71_RS03275 (KAE71_03260)	716036..716332	-	297	WP_011180278.1	HigA family addiction module antitoxin	Antitoxin
KAE71_RS03280 (KAE71_03265)	716349..716663	-	315	WP_011180277.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KAE71_RS03285 (KAE71_03270)	716726..717307	+	582	WP_011180276.1	hypothetical protein	-
KAE71_RS03290 (KAE71_03275)	717300..719228	+	1929	WP_011180275.1	phage terminase large subunit family protein	-
KAE71_RS03295 (KAE71_03280)	719232..719480	+	249	WP_011180274.1	hypothetical protein	-
KAE71_RS03300 (KAE71_03285)	719480..721036	+	1557	WP_011180273.1	phage portal protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	690729..757937	67208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (15-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-67)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12135.97 Da
• Isoelectric Point: 9.0878

>T199129 WP_011180277.1 NZ_CP072900:c716663-716349 [Bartonella henselae]
MIHKRTNEGDLVIESFADKRCKDLLEGNPPKGFPTTLVRIVQRKLFMLDKAVDLKDLRSPPGNRLEALKGERKGQYSIRI
NDQFRICFEWRSNGAYEVEIVDYH

Nucleotide

• Length: 315 bp

>T199129 NZ_CP097213:952768-953175 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11262.91 Da
• Isoelectric Point: 5.8690

>AT199129 WP_011180278.1 NZ_CP072900:c716332-716036 [Bartonella henselae]
MKNYIAIHPGEILREEYLKEYALSAYALAKALNVPRTRIERIVAENSRITPDTALRLASFFDTTAEFWLNMQAAYDVSVL
QAEKADEFSKISKFECRV

Nucleotide

• Length: 297 bp

>AT199129 NZ_CP097213:952521-952787 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0H3M2H0