Detailed information of TA system TA199099

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1595396..1596023
• Replicon: chromosome
• Accession: NZ_CP072897
• Organism: Bartonella henselae strain FR96/BK3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: X5MEM9
• Locus tag: KAE70_RS06855
• Protein ID: WP_038487056.1
• Coordinates: 1595396..1595710 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: KAE70_RS06860
• Protein ID: WP_034454130.1
• Coordinates: 1595727..1596023 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KAE70_RS06835 (KAE70_06615)	1591024..1592568	-	1545	WP_082251726.1	phage portal protein	-
KAE70_RS06840 (KAE70_06620)	1592568..1592816	-	249	WP_038487052.1	phage related protein	-
KAE70_RS06845 (KAE70_06625)	1592820..1594751	-	1932	WP_082251725.1	phage terminase large subunit family protein	-
KAE70_RS06850 (KAE70_06630)	1594782..1595333	-	552	WP_034454327.1	hypothetical protein	-
KAE70_RS06855 (KAE70_06635)	1595396..1595710	+	315	WP_038487056.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KAE70_RS06860 (KAE70_06640)	1595727..1596023	+	297	WP_034454130.1	HigA family addiction module antitoxin	Antitoxin
KAE70_RS06865 (KAE70_06645)	1596432..1596695	+	264	WP_038487057.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
KAE70_RS06870 (KAE70_06650)	1596682..1596963	+	282	WP_011180281.1	type II toxin-antitoxin system YafQ family toxin	-
KAE70_RS06875 (KAE70_06655)	1597006..1597284	-	279	WP_038487059.1	hypothetical protein	-
KAE70_RS06880 (KAE70_06660)	1597281..1598039	-	759	WP_038487061.1	antA/AntB antirepressor family protein	-
KAE70_RS06885 (KAE70_06665)	1598071..1598616	-	546	WP_236095365.1	antA/AntB antirepressor family protein	-
KAE70_RS06890 (KAE70_06670)	1598915..1599460	-	546	Protein_1344	antA/AntB antirepressor family protein	-
KAE70_RS06895 (KAE70_06675)	1600035..1600625	-	591	WP_038487063.1	antA/AntB antirepressor family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1551933..1622033	70100

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (12-104)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-80)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12135.97 Da
• Isoelectric Point: 9.0878

>T199099 WP_038487056.1 NZ_CP072897:1595396-1595710 [Bartonella henselae]
MIHKRTNEGDLVIESFADKRCKDLLEGNPPKGFPTTLVRIVQRKLFMLDKAVDLKDLRSPPGNRLDALKGERKGQYSIRI
NDQFRICFEWRSNGAYEVEIIDYH

Nucleotide

• Length: 315 bp

>T199099 NZ_CP097182:67207-67488 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11265.91 Da
• Isoelectric Point: 5.1546

>AT199099 WP_034454130.1 NZ_CP072897:1595727-1596023 [Bartonella henselae]
MKNYIAIHPGEILREEYLKEYALSAYALAKALNVPRTRIERIVAENSPVTPDTALRLAYFFDTTAEFWLNMQAAYDVSVL
QAEKADEFSKISKFECRV

Nucleotide

• Length: 297 bp

>AT199099 NZ_CP097182:66966-67217 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACTACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: X5MEM9