199066

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1765616..1766208 Replicon chromosome
Accession NZ_CP072873
Organism Pseudomonas fluorescens strain JNU01

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag J9321_RS07800 Protein ID WP_120447727.1
Coordinates 1765930..1766208 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID -
Locus tag J9321_RS07795 Protein ID WP_120447728.1
Coordinates 1765616..1765921 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J9321_RS07770 (J9321_07770) 1761442..1762359 + 918 WP_065906818.1 transcriptional regulator MetR -
J9321_RS07775 (J9321_07775) 1762374..1762787 - 414 WP_120447732.1 NUDIX domain-containing protein -
J9321_RS07780 (J9321_07780) 1762954..1763553 + 600 WP_120447731.1 helix-turn-helix transcriptional regulator -
J9321_RS07785 (J9321_07785) 1763606..1764106 + 501 WP_120447730.1 RidA family protein -
J9321_RS07790 (J9321_07790) 1764101..1765438 - 1338 WP_120447729.1 response regulator -
J9321_RS07795 (J9321_07795) 1765616..1765921 - 306 WP_120447728.1 HigA family addiction module antitoxin Antitoxin
J9321_RS07800 (J9321_07800) 1765930..1766208 - 279 WP_120447727.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J9321_RS07805 (J9321_07805) 1766374..1766646 - 273 WP_025858669.1 type B 50S ribosomal protein L31 -
J9321_RS07810 (J9321_07810) 1766747..1768072 - 1326 WP_065906826.1 APC family permease -
J9321_RS07815 (J9321_07815) 1768340..1769161 - 822 WP_120447726.1 carbon-nitrogen hydrolase family protein -
J9321_RS07820 (J9321_07820) 1769255..1770019 + 765 WP_106805031.1 LuxR C-terminal-related transcriptional regulator -
J9321_RS07825 (J9321_07825) 1769991..1770572 - 582 WP_120447725.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(13-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(17-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10518.96 Da        Isoelectric Point: 7.8618

>T199066 WP_120447727.1 NZ_CP072873:c1766208-1765930 [Pseudomonas fluorescens]
MIRSFKCADTRTVFETGRSRRWSAILSVVERKLSMLDAAVELADLRSPPGNRLEPLYGDREGQHSIRINGQFRVCFVWGP
NGPENVEIVDYH

Download         Length: 279 bp

>T199066 NZ_CP097170:c2266811-2266704 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11011.78 Da        Isoelectric Point: 6.7170

>AT199066 WP_120447728.1 NZ_CP072873:c1765921-1765616 [Pseudomonas fluorescens]
MFKNGMRPVHPGEILKEEYLEPLGLTAAALARALHVSAPTVNDIVLMRRSISADVALRLAAALETSAQFWLNLQTAYDLR
TAEIAKGDQIAKEVEKVAHCA

Download         Length: 306 bp

>AT199066 NZ_CP097170:2266859-2266925 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References