Detailed information of TA system TA199051

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 75142..75382
• Replicon: plasmid pEc184__IncF
• Accession: NZ_CP072859
• Organism: Escherichia coli strain 184/2aE

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: J9158_RS23350
• Protein ID: WP_001372321.1
• Coordinates: 75142..75267 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	75344..75382 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J9158_RS23315 (70254)	70254..70481	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
J9158_RS23320 (70569)	70569..71246	-	678	WP_001348626.1	PAS domain-containing protein	-
J9158_RS23325 (71380)	71380..71763	-	384	WP_001151566.1	conjugal transfer relaxosome DNA-binding protein TraM	-
J9158_RS23330 (72106)	72106..72696	+	591	WP_001376243.1	transglycosylase SLT domain-containing protein	-
J9158_RS23335 (72993)	72993..73814	-	822	WP_001234469.1	DUF932 domain-containing protein	-
J9158_RS23340 (73933)	73933..74220	-	288	WP_000107535.1	hypothetical protein	-
J9158_RS23345 (74245)	74245..74451	-	207	WP_000275859.1	hypothetical protein	-
J9158_RS24130 (74521)	74521..74694	+	174	Protein_91	hypothetical protein	-
J9158_RS24135 (74692)	74692..74922	-	231	WP_001426396.1	hypothetical protein	-
J9158_RS23350 (75142)	75142..75267	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
J9158_RS24140 (75209)	75209..75358	-	150	Protein_94	plasmid maintenance protein Mok	-
- (75344)	75344..75382	-	39	NuclAT_1	-	Antitoxin
- (75344)	75344..75382	-	39	NuclAT_1	-	Antitoxin
- (75344)	75344..75382	-	39	NuclAT_1	-	Antitoxin
- (75344)	75344..75382	-	39	NuclAT_1	-	Antitoxin
- (76822)	76822..76924	-	103	NuclAT_0	-	-
- (76822)	76822..76924	-	103	NuclAT_0	-	-
- (76822)	76822..76924	-	103	NuclAT_0	-	-
- (76822)	76822..76924	-	103	NuclAT_0	-	-
J9158_RS23360 (76893)	76893..77655	-	763	Protein_96	plasmid SOS inhibition protein A	-
J9158_RS23365 (77652)	77652..78086	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
J9158_RS23370 (78155)	78155..80178	-	2024	Protein_98	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / catA1 / qepA4 / dfrA12 / aadA2 / qacE / sul1 / mph(A) / erm(B)	-	1..116735	116735

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T199051 WP_001372321.1 NZ_CP072859:c75267-75142 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T199051 NZ_CP097130:2516041-2516220 [Bacillus subtilis]
ATGTCGACCTATGAATCTCTAATGGTCATGATCGGCTTTGCCAATTTAATAGGCGGGATTATGACATGGGTAATATCTCT
TTTAACATTATTATTCATGCTTAGAAAAAAAGACACTCATCCTATTTACATTACTGTAAAGGAAAAGTGTCTACACGAGG
ACCCTCCTATTAAAGGGTAG

Antitoxin

Nucleotide

• Length: 39 bp

>AT199051 NZ_CP072859:c75382-75344 [Escherichia coli]
CATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin