Detailed information of TA system TA199049

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 47175..47414
• Replicon: plasmid pEc184__IncF
• Accession: NZ_CP072859
• Organism: Escherichia coli strain 184/2aE

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: J9158_RS23190
• Protein ID: WP_023144756.1
• Coordinates: 47175..47309 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	47354..47414 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J9158_RS23160 (42876)	42876..43733	-	858	WP_000130646.1	incFII family plasmid replication initiator RepA	-
J9158_RS23165 (43726)	43726..43800	-	75	WP_014966221.1	RepA leader peptide Tap	-
J9158_RS23175 (44162)	44162..45703	+	1542	WP_002431311.1	IS21-like element ISEc12 family transposase	-
J9158_RS23180 (45718)	45718..46464	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
J9158_RS23185 (46624)	46624..46878	-	255	WP_000083850.1	replication regulatory protein RepA	-
J9158_RS23190 (47175)	47175..47309	-	135	WP_023144756.1	Hok/Gef family protein	Toxin
- (47354)	47354..47414	+	61	NuclAT_2	-	Antitoxin
- (47354)	47354..47414	+	61	NuclAT_2	-	Antitoxin
- (47354)	47354..47414	+	61	NuclAT_2	-	Antitoxin
- (47354)	47354..47414	+	61	NuclAT_2	-	Antitoxin
J9158_RS23195 (47381)	47381..47667	-	287	Protein_60	DUF2726 domain-containing protein	-
J9158_RS23200 (48180)	48180..48392	-	213	WP_013023861.1	hypothetical protein	-
J9158_RS23205 (48523)	48523..49083	-	561	WP_000139341.1	fertility inhibition protein FinO	-
J9158_RS23210 (49138)	49138..49884	-	747	WP_023909066.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / catA1 / qepA4 / dfrA12 / aadA2 / qacE / sul1 / mph(A) / erm(B)	-	1..116735	116735

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T199049 WP_023144756.1 NZ_CP072859:c47309-47175 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T199049 NZ_CP097130:521273-521623 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Nucleotide

• Length: 61 bp

>AT199049 NZ_CP072859:47354-47414 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin