Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3738707..3739248 Replicon chromosome
Accession NZ_CP072858
Organism Escherichia coli strain 184/2aE

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag J9158_RS18120 Protein ID WP_000615983.1
Coordinates 3738970..3739248 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag J9158_RS18115 Protein ID WP_000729703.1
Coordinates 3738707..3738967 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J9158_RS18095 (3734382) 3734382..3735167 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
J9158_RS18100 (3735139) 3735139..3736851 + 1713 Protein_3567 flagellar biosynthesis protein FlhA -
J9158_RS18105 (3737075) 3737075..3737572 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
J9158_RS18110 (3737748) 3737748..3738497 - 750 WP_000056849.1 C40 family peptidase -
J9158_RS18115 (3738707) 3738707..3738967 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
J9158_RS18120 (3738970) 3738970..3739248 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
J9158_RS18125 (3739404) 3739404..3740144 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
J9158_RS18130 (3740115) 3740115..3740882 - 768 WP_000333380.1 class II glutamine amidotransferase -
J9158_RS18135 (3741088) 3741088..3741666 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T199046 WP_000615983.1 NZ_CP072858:3738970-3739248 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T199046 NZ_CP097128:c890850-890446 [Conexibacter sp. DBS9H8]
ATGAGCGCTGATCGGCGGCTGTCCTACCTGGACTCCTCGGCGATCGTCAAGCTTGTGATCCAGGAGCCCGAGTCATCCGC
GCTGCGCCGCCACCTGTCGCGACGCCAGCCCGTGGTCAGCAGCGCCCTGGCCCGCACAGAGGTCGCGCGAGCGCTGCTGC
CGAGCGGACCCGAGGCTTTGGCACGGGGCGAGCAGGTTCTGCGGCGCATCCAGCTCCTACGAGTCAACGATCGCGTCCTT
GACCAAGCCGGTCCGTTGGAGCCGCCGGAGCTGCGTGCGCTCGACGCAATCCACCTGGCCAGCGCGCACCAGCTCGGAGT
GTCGGTGCGCCAGATCGTCACCTACGACCACCGTATGGCCGACGCAGCCCGCTCGCTCGGCTGGACGGTCATAGCCCCAA
GCTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT199046 WP_000729703.1 NZ_CP072858:3738707-3738967 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT199046 NZ_CP097128:c891103-890840 [Conexibacter sp. DBS9H8]
GTGGGTGTTCGGGAACTGCGACAGCGAGCCAGCGAGCTTCTGCGGCTGGTTGAGAGCGGTGAGAGCATCGAGATCACCGA
TCGTGGCCGACCGGTCGCGATACTGGGGCCGCTGCCTGAGGATCCGCTTGAGCGGTTGAGGGCGTCGGGCGATCTCCAAG
CGGCCGACGGCGAGCTCGCGGATCTTCCTGATCCGCTGCCACCTCCCGCCAGCTCGGCGCTACCGTCCTCAGTGCTCGCG
CGACTGCGGCGAGATGAGCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References