Detailed information of TA system TA199045

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3732510..3733204
• Replicon: chromosome
• Accession: NZ_CP072858
• Organism: Escherichia coli strain 184/2aE

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: J9158_RS18080
• Protein ID: WP_001263489.1
• Coordinates: 3732510..3732908 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: J9158_RS18085
• Protein ID: WP_000554758.1
• Coordinates: 3732911..3733204 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3728098)	3728098..3728178	-	81	NuclAT_12	-	-
- (3728098)	3728098..3728178	-	81	NuclAT_12	-	-
- (3728098)	3728098..3728178	-	81	NuclAT_12	-	-
- (3728098)	3728098..3728178	-	81	NuclAT_12	-	-
J9158_RS18055 (3728774)	3728774..3729232	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
J9158_RS18060 (3729493)	3729493..3730950	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
J9158_RS18065 (3731007)	3731007..3731528	-	522	Protein_3560	peptide chain release factor H	-
J9158_RS18070 (3731524)	3731524..3731730	-	207	Protein_3561	RtcB family protein	-
J9158_RS18075 (3732048)	3732048..3732500	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
J9158_RS18080 (3732510)	3732510..3732908	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
J9158_RS18085 (3732911)	3732911..3733204	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
J9158_RS18090 (3733256)	3733256..3734311	-	1056	WP_001226164.1	DNA polymerase IV	-
J9158_RS18095 (3734382)	3734382..3735167	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
J9158_RS18100 (3735139)	3735139..3736851	+	1713	Protein_3567	flagellar biosynthesis protein FlhA	-
J9158_RS18105 (3737075)	3737075..3737572	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T199045 WP_001263489.1 NZ_CP072858:c3732908-3732510 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T199045 NZ_CP097128:440373-440753 [Conexibacter sp. DBS9H8]
GTGGAGCGGCTGATTCTTGACACCTCGGTGCTGATCGACCCGGTCGATCTGCCCGAGGATGCCCAGGCGGCGATCAGCGC
GGTCTCCATCGCCGAACTGCACTTCGGGCTGCTGGTCGCCGACGAGACCACGCGCCCGTTGCGCGCCGCGCGACTCGGAC
TGATCGAGTCCCGCTTCACCCCGCTACCGCTCGATGACAAGGTCGCTCGCGAATGGGGGCGCCTCCAAGCAGCCGTCGCG
TCGCGCGACGCGAACCCGCGCAAACGCTCAGCCGACCTGGCGATCGCAGCAACAGCAAACGTTCACGAGGCGACCCTCCT
GACGCGCAACCTCAAAGACTTCACAATCATCGAGGACCTCCTACGCGTCTCGGGCGTCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT199045 WP_000554758.1 NZ_CP072858:c3733204-3732911 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT199045 NZ_CP097128:440140-440385 [Conexibacter sp. DBS9H8]
GTGACCACTATCCAGCAGAAGCAGCTCCGTAACGAGGTTGGCGATGTGCTTCGTCGTGCCGAGGCGGGAGAGGAGTTCAT
TGTCACCGTCGCCGGGCGTCCGGTTGCGCAGATCGGACCGCTGCAGCGCGACACCTGGGTACCCACGAGGCGGCTGCAGG
ATCTACTCAAGCTGCCGGCGGATCCAGCCCTGGCGGCCGACCTCGACATCTTCAAAGCCGGCTTGTCCGATCCGTGGAGC
GGCTGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3