Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 651168..651895 Replicon chromosome
Accession NZ_CP072858
Organism Escherichia coli strain 184/2aE

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag J9158_RS03180 Protein ID WP_000550189.1
Coordinates 651168..651482 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag J9158_RS03185 Protein ID WP_000560266.1
Coordinates 651479..651895 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J9158_RS03160 (647335) 647335..648321 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
J9158_RS03165 (648400) 648400..649083 - 684 WP_001183043.1 vancomycin high temperature exclusion protein -
J9158_RS03170 (649160) 649160..649663 - 504 WP_001295542.1 M48 family metallopeptidase -
J9158_RS03175 (649748) 649748..650884 + 1137 WP_000018681.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
J9158_RS03180 (651168) 651168..651482 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
J9158_RS03185 (651479) 651479..651895 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
J9158_RS03190 (651940) 651940..653958 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
J9158_RS03195 (654384) 654384..656735 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T199026 WP_000550189.1 NZ_CP072858:651168-651482 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T199026 NZ_CP097107:c4565703-4565329 [Citrobacter freundii]
ATGGTCAAAGGATCCGCGCTTTTTGATACCAACATCCTCATCGATCTGTTTAGTGGGAGAAATGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAATGCAATCAGCCTCATTACATGGATGGAGGTGATGGTGGGTGCAAAAAAATACCATCAGG
AAGCCCGTACTCGTGTTGCGCTAGGTGCTTTTAACGTGATCGGCGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGACAGGAATATGGGATGAAATTGCCGGATGCCATTATTTTGGCAACAGCACAGATTCATCGTCTTACGCTGGTTACGCG
AAATACTAAGGATTTTGCAGGCCTATCCGGTGTGGTCACACCGTATACATTATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT199026 WP_000560266.1 NZ_CP072858:651479-651895 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT199026 NZ_CP097107:c4565945-4565703 [Citrobacter freundii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAAGTGATTAAACGTCTTGATGATCTCAAGGT
GCAGCGCAACCTGCCGCGAGCAGAACTTTTACGGGAGGCCGTTGAACAGTATCTTGAGAGGCAGGATCAGGCTAAAACCA
CAATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTCCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References