Detailed information of TA system TA198997

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 2401017..2401615
• Replicon: chromosome
• Accession: NZ_CP072849
• Organism: Vibrio cholerae MO10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q8KQX9
• Locus tag: KAF59_RS10865
• Protein ID: WP_000118545.1
• Coordinates: 2401017..2401340 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KAF59_RS10870
• Protein ID: WP_000058216.1
• Coordinates: 2401337..2401615 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KAF59_RS10835 (KAF59_10835)	2396730..2397116	-	387	WP_000180225.1	TraA family conjugative transfer protein	-
KAF59_RS10840 (KAF59_10840)	2397113..2397685	-	573	WP_139057167.1	type IV conjugative transfer system lipoprotein TraV	-
KAF59_RS10845 (KAF59_10845)	2397760..2399049	-	1290	WP_001883765.1	TraB/VirB10 family protein	-
KAF59_RS10850 (KAF59_10850)	2399052..2399948	-	897	WP_032467354.1	type-F conjugative transfer system secretin TraK	-
KAF59_RS10855 (KAF59_10855)	2399932..2400558	-	627	WP_000667169.1	TraE/TraK family type IV conjugative transfer system protein	-
KAF59_RS10860 (KAF59_10860)	2400555..2400836	-	282	WP_000433889.1	type IV conjugative transfer system protein TraL	-
KAF59_RS10865 (KAF59_10865)	2401017..2401340	+	324	WP_000118545.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KAF59_RS10870 (KAF59_10870)	2401337..2401615	+	279	WP_000058216.1	helix-turn-helix transcriptional regulator	Antitoxin
KAF59_RS10875 (KAF59_10875)	2401641..2402276	-	636	WP_000033753.1	DUF4400 domain-containing protein	-
KAF59_RS10880 (KAF59_10880)	2402263..2402823	-	561	WP_000546510.1	hypothetical protein	-
KAF59_RS10885 (KAF59_10885)	2402833..2404653	-	1821	WP_000661887.1	conjugative transfer system coupling protein TraD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2309421..2442312	132891

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12267.26 Da
• Isoelectric Point: 9.4705

>T198997 WP_000118545.1 NZ_CP072849:2401017-2401340 [Vibrio cholerae MO10]
MSWKIDFYDGVEDQILDMPPKIQARMIKLLELMEKHGANLGPPHTESMGDGLFEIRAKAQEGIGRGLFCYLKGNHIYVLH
AFVKKSQKTPKNELNLARDRQKEVQKS

Nucleotide

• Length: 324 bp

>T198997 NZ_CP097082:c4675218-4674934 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10517.07 Da
• Isoelectric Point: 9.9393

>AT198997 WP_000058216.1 NZ_CP072849:2401337-2401615 [Vibrio cholerae MO10]
MSLQKLKQRALANAEVKNEYDKLESEFSFIDQLLTMRTKAGLTQEQVAERMHTQKSNVSRLEKGNANPSWSTLLKYAHAC
GFELTLKPQKMS

Nucleotide

• Length: 279 bp

>AT198997 NZ_CP097082:c4675450-4675208 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A0X1L383