Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2498154..2498751 Replicon chromosome
Accession NZ_CP072826
Organism Klebsiella pneumoniae strain IRQBAS105c

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag J9263_RS12150 Protein ID WP_004142563.1
Coordinates 2498154..2498471 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag J9263_RS12155 Protein ID WP_004142561.1
Coordinates 2498464..2498751 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J9263_RS12135 (2493858) 2493858..2495285 - 1428 WP_004176980.1 MFS transporter -
J9263_RS12140 (2495394) 2495394..2496260 + 867 WP_048299254.1 helix-turn-helix transcriptional regulator -
J9263_RS12145 (2496926) 2496926..2497969 + 1044 WP_135707754.1 DUF2157 domain-containing protein -
J9263_RS12150 (2498154) 2498154..2498471 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J9263_RS12155 (2498464) 2498464..2498751 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
J9263_RS12160 (2499016) 2499016..2499669 - 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
J9263_RS12165 (2499789) 2499789..2500157 - 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
J9263_RS12170 (2500157) 2500157..2500738 - 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
J9263_RS12175 (2500918) 2500918..2502033 + 1116 WP_012737592.1 MBL fold metallo-hydrolase -
J9263_RS12180 (2502064) 2502064..2502405 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
J9263_RS12185 (2502423) 2502423..2502671 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T198947 WP_004142563.1 NZ_CP072826:2498154-2498471 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T198947 NZ_CP097067:c47955-47614 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGAAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACATTGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCAACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGACTTTGCAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT198947 WP_004142561.1 NZ_CP072826:2498464-2498751 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT198947 NZ_CP097067:c48185-47955 [Enterococcus faecalis]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGACCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References