Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 3004996..3005582 Replicon chromosome
Accession NZ_CP072825
Organism Klebsiella pneumoniae strain IRQBAS103c

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag J9262_RS14700 Protein ID WP_002920800.1
Coordinates 3005214..3005582 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag J9262_RS14695 Protein ID WP_004174006.1
Coordinates 3004996..3005217 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J9262_RS14675 (3001153) 3001153..3002079 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
J9262_RS14680 (3002076) 3002076..3003353 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
J9262_RS14685 (3003350) 3003350..3004117 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
J9262_RS14690 (3004119) 3004119..3004832 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
J9262_RS14695 (3004996) 3004996..3005217 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
J9262_RS14700 (3005214) 3005214..3005582 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
J9262_RS14705 (3005856) 3005856..3007172 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
J9262_RS14710 (3007279) 3007279..3008166 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
J9262_RS14715 (3008163) 3008163..3009008 + 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
J9262_RS14720 (3009010) 3009010..3010080 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3002076..3010817 8741


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T198933 WP_002920800.1 NZ_CP072825:3005214-3005582 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T198933 NZ_CP097061:184548-184814 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATATTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGCGGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT198933 WP_004174006.1 NZ_CP072825:3004996-3005217 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT198933 NZ_CP097061:184285-184548 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTAGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTGGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References