Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3688115..3688842 Replicon chromosome
Accession NZ_CP072802
Organism Escherichia coli strain PK5074

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag J8617_RS17885 Protein ID WP_000550189.1
Coordinates 3688115..3688429 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag J8617_RS17890 Protein ID WP_000560266.1
Coordinates 3688426..3688842 (+) Length 139 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8617_RS17865 (3684273) 3684273..3685259 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
J8617_RS17870 (3685338) 3685338..3686030 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
J8617_RS17875 (3686107) 3686107..3686610 - 504 WP_001333820.1 M48 family metallopeptidase -
J8617_RS17880 (3686695) 3686695..3687831 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
J8617_RS17885 (3688115) 3688115..3688429 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
J8617_RS17890 (3688426) 3688426..3688842 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
J8617_RS17895 (3688887) 3688887..3690905 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
J8617_RS17900 (3691331) 3691331..3693682 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T198909 WP_000550189.1 NZ_CP072802:3688115-3688429 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T198909 NZ_CP097056:c2644525-2644382 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACAGAAAGGAGAGGCCTGTTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGG
TATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAATAAAAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT198909 WP_000560266.1 NZ_CP072802:3688426-3688842 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT198909 NZ_CP097056:2644263-2644449 [Enterococcus faecalis]
TGTGCTATAATGAAAACGAAAAGAGAGATATGCGTCAACATACCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCGATT
TTGTTACAAAAAATAACCGTACTCGATCAAAGTAAACGGTTATTTTTTATTGTCATTTTTAAGCAATTTCACAATCAGCG
CAATCAAAGCAATGGTAAACATACCAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML


Antitoxin

Source ID Structure

References