Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3644770..3645569 Replicon chromosome
Accession NZ_CP072802
Organism Escherichia coli strain PK5074

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag J8617_RS17665 Protein ID WP_000347273.1
Coordinates 3644770..3645234 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag J8617_RS17670 Protein ID WP_001307405.1
Coordinates 3645234..3645569 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8617_RS17635 (3639771) 3639771..3640205 - 435 WP_000948842.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
J8617_RS17640 (3640223) 3640223..3641101 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
J8617_RS17645 (3641091) 3641091..3641870 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
J8617_RS17650 (3641881) 3641881..3642354 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
J8617_RS17655 (3642377) 3642377..3643657 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
J8617_RS17660 (3643906) 3643906..3644715 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
J8617_RS17665 (3644770) 3644770..3645234 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
J8617_RS17670 (3645234) 3645234..3645569 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
J8617_RS17675 (3645718) 3645718..3647289 - 1572 WP_001273753.1 galactarate dehydratase -
J8617_RS17680 (3647664) 3647664..3648998 + 1335 WP_210388628.1 MFS transporter -
J8617_RS17685 (3649014) 3649014..3649784 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T198908 WP_000347273.1 NZ_CP072802:c3645234-3644770 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T198908 NZ_CP097056:c2570020-2569919 [Enterococcus faecalis]
TTGTCAATCGAAGCGACATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACGATAAAAAATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT198908 WP_001307405.1 NZ_CP072802:c3645569-3645234 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT198908 NZ_CP097056:2569794-2569974 [Enterococcus faecalis]
TGAAAAGAGAGGTATGCGGGTACATACCTCTCCTTTTATACCAACACCAGTTATAAGAACTGGTGGCTTACTGAGTTAAG
TTTTTGTTGTTCTTTAACCGTTCAGCCGTCTAAGCTTATGAACGGTTATTTTTTATCGTTTTTCGTTGCTTCAAGGATAC
CGAAAATCAGTAGTGCAACAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References