Detailed information of TA system TA198904

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 2034269..2034810
• Replicon: chromosome
• Accession: NZ_CP072802
• Organism: Escherichia coli strain PK5074

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: Q32JN3
• Locus tag: J8617_RS09965
• Protein ID: WP_000615980.1
• Coordinates: 2034532..2034810 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: J8617_RS09960
• Protein ID: WP_000729707.1
• Coordinates: 2034269..2034529 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8617_RS09940 (2029935)	2029935..2030720	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
J8617_RS09945 (2030692)	2030692..2032404	+	1713	Protein_1976	flagellar biosynthesis protein FlhA	-
J8617_RS09950 (2032637)	2032637..2033134	-	498	WP_000006245.1	REP-associated tyrosine transposase RayT	-
J8617_RS09955 (2033310)	2033310..2034083	-	774	WP_000543885.1	C40 family peptidase	-
J8617_RS09960 (2034269)	2034269..2034529	+	261	WP_000729707.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
J8617_RS09965 (2034532)	2034532..2034810	+	279	WP_000615980.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
J8617_RS09970 (2034966)	2034966..2035706	+	741	WP_001225679.1	peptidoglycan meso-diaminopimelic acid protein amidase	-
J8617_RS09975 (2035677)	2035677..2036444	-	768	WP_000333380.1	class II glutamine amidotransferase	-
J8617_RS09980 (2036650)	2036650..2037228	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (5-92)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10830.62 Da
• Isoelectric Point: 10.0702

>T198904 WP_000615980.1 NZ_CP072802:2034532-2034810 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T198904 NZ_CP097056:c130279-129641 [Enterococcus faecalis]
ATGATACTAAATAAGAAAAAATTATATTTCGAAACACAGGCATTTACTGACAAACTTATAAATGAAATTTCAATCAATAT
GTCTAAAAGAAAAGAACTCATCAATTGTTATGATATTGAAGCCTACGTAAAAGAAACCGAAAACATTGATTTTTTTGATT
ATCCATTCAAAAAACAATCTAAAAAATTAATCTGGGGATCAGTAACAAAAATTGGTGATGTAACTATGGTAGCGACTGCC
CAACACTTGAAAATAGAAATTAAAAATTTTACGAAAATGCACGAAATAATTCATCATTATTTTGACTATACGACAGATAT
GCCTAACAATGAAAAATCATTTGATACACTACTTATAAAAAAGGGCTATCTTCCTGAAGATTATCCTAAAGAATTCAGAG
CAAATAAGGGTGCCGGAATGTTACTTATTAATAAAAAAGCCTTAAAGTATGCATTTAGTACTTATGATGATCTTGAAAAA
ATGGCTAGTTTTTTCAACGTAAACGATATAGTTCTCAAGATAAGAATTCTTGACGAATTAATCTATTCCCACAACTTATC
TTATAAACAAGCCAATCTTATTTATAATAGTTACTATTTTCAACAACAAACACAACTTAAAGAGATTTTACTCAAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9421.80 Da
• Isoelectric Point: 5.0758

>AT198904 WP_000729707.1 NZ_CP072802:2034269-2034529 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKTKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT198904 NZ_CP097056:c130660-130286 [Enterococcus faecalis]
ATGAAAACTTACGAAAAGATAAAACAATTAAGAGAAAATAGAGAAATCACTCAAAAAGAAATTTCAAACGCACTAGACAT
TAATGTTAGTGTATATAATAAAATAGAACTTGGAATACGTCCATTAAGAGAAGAAGAACTTACTGCTATTGCTGATTTTT
TTAATGTTTCAATTGATTATTTAACAGGACGCACTGATAACCCTACAACACCTTCAAATAATCAAAAAGAAGCTGGACAA
AATATTATTTCACATTTCAGATTAAACACTAATGATTTAAGTTCTGATGACATAGAAGAATTAGAAAAAGAATTGATTGA
TTTTCAAGAATTTCTTATAAAACGAGCAAAAGAAAGAAAAAAAAAGAGTGAGTAA

Structures

Toxin

• AlphaFold DB: A0A090NH48