Detailed information of TA system TA198884

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1546664..1547436
• Replicon: chromosome
• Accession: NZ_CP072801
• Organism: Thiothrix litoralis strain AS

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: J9253_RS07490
• Protein ID: WP_228291536.1
• Coordinates: 1546664..1546978 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J9253_RS07495
• Protein ID: WP_210223996.1
• Coordinates: 1546978..1547436 (+)
• Length: 153 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J9253_RS07470 (J9253_07470)	1541759..1542016	+	258	WP_210223993.1	nitrogen fixation protein NifZ	-
J9253_RS07475 (J9253_07475)	1542168..1544426	-	2259	WP_210223994.1	DUF262 domain-containing protein	-
J9253_RS07480 (J9253_07480)	1544652..1545239	-	588	WP_228291535.1	Uma2 family endonuclease	-
J9253_RS07485 (J9253_07485)	1545313..1546179	+	867	WP_210223995.1	ATP-binding cassette domain-containing protein	-
J9253_RS07490 (J9253_07490)	1546664..1546978	+	315	WP_228291536.1	type II toxin-antitoxin system HigB family toxin	Toxin
J9253_RS07495 (J9253_07495)	1546978..1547436	+	459	WP_210223996.1	transcriptional regulator	Antitoxin
J9253_RS07500 (J9253_07500)	1548403..1549782	-	1380	WP_210223997.1	hypothetical protein	-
J9253_RS07505 (J9253_07505)	1549775..1551121	-	1347	WP_210221796.1	IS4 family transposase	-
J9253_RS07510 (J9253_07510)	1551135..1551644	-	510	WP_210223998.1	hypothetical protein	-
J9253_RS07515 (J9253_07515)	1551655..1552002	-	348	WP_210223999.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-94)

Antitoxin

• Antitoxin HigA (8-139)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12120.83 Da
• Isoelectric Point: 8.8482

>T198884 WP_228291536.1 NZ_CP072801:1546664-1546978 [Thiothrix litoralis]
VLHVISRKPFNEASLNFPNDADAIDAAYKTLRNGSFANPLELKAVFPSLDNFRYKDKWWVIDIGGNNLRLLAFIEFRDNR
MYVKHIVTHADYDKLCKKYAREAD

Nucleotide

• Length: 315 bp

>T198884 NZ_CP097046:c2533456-2533355 [Enterococcus faecalis]
TTGTCAATCGAAGCGGCATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACAATAAAAAATAA

Antitoxin

Protein

• Length: 153 a.a.
• Molecular weight: 17278.73 Da
• Isoelectric Point: 4.5343

>AT198884 WP_210223996.1 NZ_CP072801:1546978-1547436 [Thiothrix litoralis]
MDFAAVKIKAKELFAQTSFLTEIRDDIDYENALALMEELLEDYDEQRTLIGILANTIEEWENVAPAFAEFNQRVAKLDDG
VAVLRTLIDQYQLKAEDLKEEIGSKSLVSMILNGSRNLTREHIQALSLRFKLNPAIFFHTTALRLVSTSRTA

Nucleotide

• Length: 459 bp

>AT198884 NZ_CP097046:2533236-2533410 [Enterococcus faecalis]
AAAAGAGAGGTATGCGGGTACATACCTCTCCTTTTATACCAACACCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGTT
TTGAATCTTAACCGTTCGGCCTACGAAGCTTGTGAACGGTTATTTTTTATTGTTTTTCGTTGCTTCAAGGATACCGAAAA
TCAGTAGTGCAACAA