Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3543619..3544289 Replicon chromosome
Accession NZ_CP072790
Organism Mycobacterium tuberculosis strain I0003447-5

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag J8397_RS16825 Protein ID WP_003899954.1
Coordinates 3543619..3543963 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag J8397_RS16830 Protein ID WP_003899955.1
Coordinates 3543960..3544289 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8397_RS16790 (J8397_16595) 3538692..3539552 + 861 WP_003416628.1 alpha/beta hydrolase -
J8397_RS16795 (J8397_16600) 3539527..3540042 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
J8397_RS16800 3540058..3540269 + 212 Protein_3318 (R)-hydratase -
J8397_RS16805 (J8397_16605) 3540282..3540575 + 294 WP_003416635.1 hypothetical protein -
J8397_RS16810 (J8397_16610) 3540863..3542152 + 1290 WP_003416640.1 ATP-binding protein -
J8397_RS16815 (J8397_16615) 3542499..3542933 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
J8397_RS16820 (J8397_16620) 3542936..3543388 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
J8397_RS16825 (J8397_16625) 3543619..3543963 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J8397_RS16830 (J8397_16630) 3543960..3544289 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
J8397_RS16835 (J8397_16635) 3544827..3545174 + 348 WP_003899956.1 DUF2384 domain-containing protein -
J8397_RS16840 (J8397_16640) 3545171..3545791 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
J8397_RS16845 (J8397_16645) 3545951..3547216 - 1266 WP_003902423.1 hypothetical protein -
J8397_RS16850 (J8397_16650) 3547384..3547593 + 210 WP_003416778.1 hypothetical protein -
J8397_RS16855 (J8397_16655) 3547840..3548874 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T198855 WP_003899954.1 NZ_CP072790:3543619-3543963 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T198855 NZ_CP097032:c67535-67194 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGTTGGAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACAATGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCCACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGATTTTACAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT198855 WP_003899955.1 NZ_CP072790:3543960-3544289 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT198855 NZ_CP097032:c67765-67535 [Enterococcus faecalis]
ATGTTAACAACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGATCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References