Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2262236..2263155 Replicon chromosome
Accession NZ_CP072790
Organism Mycobacterium tuberculosis strain I0003447-5

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag J8397_RS10675 Protein ID WP_003900449.1
Coordinates 2262550..2263155 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag J8397_RS10670 Protein ID WP_003410124.1
Coordinates 2262236..2262541 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8397_RS10640 (J8397_10505) 2257247..2258503 - 1257 WP_003410095.1 HNH endonuclease signature motif containing protein -
J8397_RS10645 (J8397_10510) 2258857..2259432 + 576 WP_003410100.1 hypothetical protein -
J8397_RS10650 (J8397_10515) 2259429..2260469 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
J8397_RS10655 (J8397_10520) 2260711..2261430 + 720 WP_003410108.1 DUF433 domain-containing protein -
J8397_RS10660 (J8397_10525) 2261420..2261836 + 417 WP_003410114.1 hypothetical protein -
J8397_RS10665 (J8397_10530) 2261852..2262151 - 300 WP_003410120.1 hypothetical protein -
J8397_RS10670 (J8397_10535) 2262236..2262541 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
J8397_RS10675 (J8397_10540) 2262550..2263155 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J8397_RS10680 (J8397_10545) 2263180..2263539 - 360 WP_003410131.1 hypothetical protein -
J8397_RS10685 (J8397_10550) 2263699..2264289 - 591 WP_057145698.1 hypothetical protein -
J8397_RS10690 (J8397_10555) 2264328..2264564 - 237 WP_003410133.1 hypothetical protein -
J8397_RS10695 (J8397_10560) 2264771..2265667 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T198837 WP_003900449.1 NZ_CP072790:c2263155-2262550 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T198837 NZ_CP097030:2680983-2681105 [Enterococcus faecalis]
ATGAAAGGAGTAGGCCTTTTGTCAGCATATGAGACAATTCAGACGATCCTAGGGTTTGGTATGTTTACCATTGCTTTGAT
TGCACTGATTGTGAAATTGCTTAAAGAAGACAAAAAGAAATGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT198837 WP_003410124.1 NZ_CP072790:c2262541-2262236 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT198837 NZ_CP097030:c2681245-2681038 [Enterococcus faecalis]
TTTTTAGGTAATTGTGCTATAATAGCAATGAAAAGAGAGATATGCTGTAACATACCTCTCTAGTGTAGAGCCGTTACTAA
TGGTGACAAAATTTTAAAGATTTTGAAATGACCGTCTAACTAGGCAAAGTTAAACAGCGGTCATTTCTTTTTGTCTTCTT
TAAGCAATTTCACAATCAGTGCAATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References