Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1162674..1163368 Replicon chromosome
Accession NZ_CP072786
Organism Escherichia coli strain JA0257

Toxin (Protein)


Gene name yafO Uniprot ID B7MQ69
Locus tag I0P57_RS05410 Protein ID WP_001263501.1
Coordinates 1162970..1163368 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag I0P57_RS05405 Protein ID WP_000554755.1
Coordinates 1162674..1162967 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I0P57_RS05385 (1158388) 1158388..1158960 + 573 WP_000086540.1 recombinase family protein -
I0P57_RS05390 (1158964) 1158964..1159689 - 726 WP_226226728.1 hypothetical protein -
I0P57_RS05395 (1159964) 1159964..1161508 - 1545 WP_000758648.1 recombinase family protein -
I0P57_RS05400 (1161570) 1161570..1162622 + 1053 WP_000691316.1 DNA polymerase IV -
I0P57_RS05405 (1162674) 1162674..1162967 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
I0P57_RS05410 (1162970) 1162970..1163368 + 399 WP_001263501.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
I0P57_RS05415 (1163378) 1163378..1163830 + 453 WP_001059897.1 GNAT family N-acetyltransferase -
I0P57_RS05420 (1164020) 1164020..1165159 + 1140 WP_000521578.1 RNA ligase RtcB family protein -
I0P57_RS05425 (1165156) 1165156..1165770 + 615 WP_000602123.1 peptide chain release factor H -
I0P57_RS05430 (1165827) 1165827..1167284 - 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
I0P57_RS05435 (1167545) 1167545..1168003 + 459 WP_001291991.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0950

>T198755 WP_001263501.1 NZ_CP072786:1162970-1163368 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T198755 NZ_CP096976:1987861-1988112 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT198755 WP_000554755.1 NZ_CP072786:1162674-1162967 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT198755 NZ_CP096976:1988109-1988363 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376DBU4

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References