Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2457722..2458275 Replicon chromosome
Accession NZ_CP072785
Organism Escherichia coli strain JA0265

Toxin (Protein)


Gene name higB Uniprot ID A0A0G9FUJ7
Locus tag I0P60_RS11765 Protein ID WP_000605680.1
Coordinates 2458006..2458275 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag I0P60_RS11760 Protein ID WP_000781370.1
Coordinates 2457722..2458006 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I0P60_RS11745 (2452903) 2452903..2455950 + 3048 WP_077634977.1 formate dehydrogenase-N subunit alpha -
I0P60_RS11750 (2455963) 2455963..2456847 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
I0P60_RS11755 (2456840) 2456840..2457493 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
I0P60_RS11760 (2457722) 2457722..2458006 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
I0P60_RS11765 (2458006) 2458006..2458275 - 270 WP_000605680.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I0P60_RS11770 (2458461) 2458461..2459471 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
I0P60_RS11775 (2459605) 2459605..2461302 - 1698 WP_000433464.1 malate dehydrogenase -
I0P60_RS11780 (2461459) 2461459..2461596 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
I0P60_RS11785 (2461698) 2461698..2461913 - 216 WP_000495766.1 biofilm-dependent modulation protein -
I0P60_RS11790 (2462258) 2462258..2462689 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-89)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10181.73 Da        Isoelectric Point: 6.8582

>T198734 WP_000605680.1 NZ_CP072785:c2458275-2458006 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDA
YILDYEDYH

Download         Length: 270 bp

>T198734 NZ_CP096971:c3153548-3153153 [Sphingomonas sp. SUN019]
ATGATCGTCGACACGTCGGCGCTGATGGCGATTATCTTGGGCGAACCCGAACGCGATCTTTTCATCGCGATATTGGCTTC
GGCGGACACGTTATCGATATCGGCGGGAAGTTGGGTCGAACTCGGTGCCGTGATCGTCCGCAGGCAACAGCACATGTTGG
AACACGAATTACTGGCATTGCGCGACGATTTCGAGTTTGGGGTCGAACCGGTGACGGTCATCCAAGCCGATTTGGCTCGC
GTCGCTTACAGAGAATACGGTCGCGGAACCGGCCACCCCGCCAAACTAAACTTCGGCGATTGCTTCGCCTACGCGCTCGC
CAGGGAAACCGGCGAACCGCTTCTCTTCAAGGGAAACGATTTCTCACAGACCGATATCAATCCCGCGGTGAAGTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT198734 WP_000781370.1 NZ_CP072785:c2458006-2457722 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT198734 NZ_CP096971:c3153811-3153545 [Sphingomonas sp. SUN019]
ATGGGCGTTCAACTTAATATCAAAGATCCCGAGACGGTACGCCTCGCGCGCGAACTGGCGGGTGACACGGGGCGAACGGT
CACGGAAACGGTTCGCGCTGCCCTGCAACGTGCGCATGAAGATCGTGCAGATAAGATCCAGGCGAAGATCGCCAAAATGA
ACGCGGCGGTCGACCGACTTCGCAAGCATCTACCGCCCGAATGGAAAGGTCGTACCTCAAAAGAAATAATGGACGAGCTG
TATGACGAGGACGGCCTGCCCATATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FUJ7

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References