Detailed information of TA system TA198701

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 172242..172844
• Replicon: chromosome
• Accession: NZ_CP072784
• Organism: Escherichia coli strain JA0266

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: I0P58_RS00825
• Protein ID: WP_000897302.1
• Coordinates: 172242..172553 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I0P58_RS00830
• Protein ID: WP_000356397.1
• Coordinates: 172554..172844 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I0P58_RS00800 (168156)	168156..168755	+	600	WP_001314324.1	glucose-1-phosphatase	-
I0P58_RS00805 (168749)	168749..169621	+	873	WP_000920762.1	virulence factor BrkB family protein	-
I0P58_RS00810 (169618)	169618..170055	+	438	WP_000560990.1	D-aminoacyl-tRNA deacylase	-
I0P58_RS00815 (170100)	170100..171041	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I0P58_RS00820 (171105)	171105..172013	-	909	WP_001331553.1	alpha/beta hydrolase	-
I0P58_RS00825 (172242)	172242..172553	+	312	WP_000897302.1	hypothetical protein	Toxin
I0P58_RS00830 (172554)	172554..172844	+	291	WP_000356397.1	NadS family protein	Antitoxin
I0P58_RS00835 (173203)	173203..173481	+	279	WP_001296612.1	hypothetical protein	-
I0P58_RS00840 (173877)	173877..174095	+	219	WP_001314326.1	CopG family transcriptional regulator	-
I0P58_RS00845 (174311)	174311..175240	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
I0P58_RS00850 (175237)	175237..175872	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I0P58_RS00855 (175869)	175869..176771	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T198701 WP_000897302.1 NZ_CP072784:172242-172553 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T198701 NZ_CP096957:217390-217716 [Pseudomonas aeruginosa]
ATGATGCGCGGCGACCTTGTGACCATCGCCATGCAGGGCGACTTTGGCAAGCCACGGCCTGCGCTTGTGGTTCAGGCCAA
CCAGTTCAGCGAGCACGGCAGCGTTACCGTGCTGCCTGTCACCAGCACACTTAATGCCGCGCCGTTGCTGCGCGTCACGG
TTCAGCCGAGCGCAGAGAACGGTTTGCAGAAGCCATCGCAGGTGATGGTCGACAAGGCGATGACGGTGCTGCGCAGCAAG
GTAGGGCCAGCTTTCGGGCGCATCGACGCGGACGCACTGCTTGAGGTTGAGCGTTGCTTGGCCGTGTTCTTGGGGATTGC
GAAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT198701 WP_000356397.1 NZ_CP072784:172554-172844 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT198701 NZ_CP096957:217169-217393 [Pseudomonas aeruginosa]
ATGGCAACAGCACACGTTAATCAGCGGGTTCAGAAGCATCGCGATGCATTACGCATGGCGGGGCTTCGTCCGGTTCAAAT
CTGGGTGCCGGATACGCGCCGCCCCGACTTCGCGGAGGAGTGCCGCCGCCAATGCCTGCTTGTGGCTCAGGCCGACAGCG
CCGACACCGCCATGCAGCAGTTCATGGATGAAGCCTTGGCAGACGTGGACGGCTGGACGGAATGA

Structures

Toxin

• AlphaFold DB: S1P416