Detailed information of TA system TA198692

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3390956..3391554
• Replicon: chromosome
• Accession: NZ_CP072779
• Organism: Proteus mirabilis strain 1035

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: J8N15_RS15830
• Protein ID: WP_210234517.1
• Coordinates: 3390956..3391279 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J8N15_RS15835
• Protein ID: WP_000058216.1
• Coordinates: 3391276..3391554 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8N15_RS15800 (J8N15_15800)	3386669..3387055	-	387	WP_000180225.1	TraA family conjugative transfer protein	-
J8N15_RS15805 (J8N15_15805)	3387052..3387624	-	573	WP_139175592.1	type IV conjugative transfer system lipoprotein TraV	-
J8N15_RS15810 (J8N15_15810)	3387699..3388988	-	1290	WP_210234515.1	TraB/VirB10 family protein	-
J8N15_RS15815 (J8N15_15815)	3388991..3389887	-	897	WP_210234582.1	type-F conjugative transfer system secretin TraK	-
J8N15_RS15820 (J8N15_15820)	3389871..3390497	-	627	WP_210234516.1	TraE/TraK family type IV conjugative transfer system protein	-
J8N15_RS15825 (J8N15_15825)	3390494..3390775	-	282	WP_000433891.1	type IV conjugative transfer system protein TraL	-
J8N15_RS15830 (J8N15_15830)	3390956..3391279	+	324	WP_210234517.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J8N15_RS15835 (J8N15_15835)	3391276..3391554	+	279	WP_000058216.1	helix-turn-helix transcriptional regulator	Antitoxin
J8N15_RS15840 (J8N15_15840)	3391580..3392215	-	636	WP_210234518.1	DUF4400 domain-containing protein	-
J8N15_RS15845 (J8N15_15845)	3392202..3392762	-	561	WP_210234519.1	conjugative transfer protein	-
J8N15_RS15850 (J8N15_15850)	3392772..3394607	-	1836	WP_210234520.1	conjugative transfer system coupling protein TraD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(J) / sul2 / aph(3'')-Ib / aph(3')-VIb / blaPER-1 / aph(6)-Id / aac(3)-IId / erm(42)	-	3292765..3481590	188825

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (15-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12294.41 Da
• Isoelectric Point: 9.7658

>T198692 WP_210234517.1 NZ_CP072779:3390956-3391279 [Proteus mirabilis]
MSWKIDFYDGVEEQILDMPPKIQARMIKLLELMEKHGANLGPPHTESMGDGLFEIRAKAQEGIGRGLFCYLKGKHIYVLH
AFVKKSQKTPKIELNLARDRQKEVQKS

Nucleotide

• Length: 324 bp

>T198692 NZ_CP096953:c5782557-5782279 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10517.07 Da
• Isoelectric Point: 9.9393

>AT198692 WP_000058216.1 NZ_CP072779:3391276-3391554 [Proteus mirabilis]
MSLQKLKQRALANAEVKNEYDKLESEFSFIDQLLTMRTKAGLTQEQVAERMHTQKSNVSRLEKGNANPSWSTLLKYAHAC
GFELTLKPQKMS

Nucleotide

• Length: 279 bp

>AT198692 NZ_CP096953:c5782267-5781962 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG