Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2981583..2982231 Replicon chromosome
Accession NZ_CP072765
Organism Mycobacterium tuberculosis strain H37Rv_CG

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag J8668_RS13940 Protein ID WP_003899414.1
Coordinates 2981583..2981906 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag J8668_RS13945 Protein ID WP_003899415.1
Coordinates 2981986..2982231 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8668_RS13920 (J8668_13920) 2977157..2978418 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
J8668_RS13925 (J8668_13925) 2978792..2980231 - 1440 WP_009935349.1 phage major capsid protein -
J8668_RS13930 (J8668_13930) 2980239..2980772 - 534 WP_003899412.1 HK97 family phage prohead protease -
J8668_RS13935 (J8668_13935) 2980925..2981416 - 492 WP_003900541.1 phage terminase small subunit P27 family -
J8668_RS13940 (J8668_13940) 2981583..2981906 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
J8668_RS13945 (J8668_13945) 2981986..2982231 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
J8668_RS13950 (J8668_13950) 2982228..2983655 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
J8668_RS13955 (J8668_13955) 2983657..2984049 - 393 WP_003899417.1 DUF2742 domain-containing protein -
J8668_RS13960 (J8668_13960) 2984046..2984306 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
J8668_RS13965 (J8668_13965) 2984323..2984685 - 363 WP_003900543.1 hypothetical protein -
J8668_RS13970 (J8668_13970) 2984688..2985815 - 1128 WP_003899420.1 site-specific integrase -
J8668_RS13975 (J8668_13975) 2985960..2986187 - 228 WP_003899421.1 hypothetical protein -
J8668_RS13980 (J8668_13980) 2986184..2986573 - 390 WP_003899422.1 hypothetical protein -
J8668_RS13985 (J8668_13985) 2986479..2986751 + 273 WP_003900544.1 hypothetical protein -
J8668_RS13990 (J8668_13990) 2986850..2987083 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2975548..2985815 10267


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(22-69)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T198671 WP_003899414.1 NZ_CP072765:c2981906-2981583 [Mycobacterium tuberculosis]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T198671 NZ_CP096945:c5682420-5682142 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT198671 WP_003899415.1 NZ_CP072765:c2982231-2981986 [Mycobacterium tuberculosis]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT198671 NZ_CP096945:c5682130-5681825 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References