Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2269736..2270655 Replicon chromosome
Accession NZ_CP072765
Organism Mycobacterium tuberculosis strain H37Rv_CG

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag J8668_RS10580 Protein ID WP_003900449.1
Coordinates 2270050..2270655 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag J8668_RS10575 Protein ID WP_003410124.1
Coordinates 2269736..2270041 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8668_RS10545 (J8668_10545) 2264747..2266003 - 1257 WP_003410095.1 HNH endonuclease signature motif containing protein -
J8668_RS10550 (J8668_10550) 2266357..2266932 + 576 WP_003410100.1 hypothetical protein -
J8668_RS10555 (J8668_10555) 2266929..2267969 + 1041 WP_003911735.1 ImmA/IrrE family metallo-endopeptidase -
J8668_RS10560 (J8668_10560) 2268211..2268930 + 720 WP_003410108.1 DUF433 domain-containing protein -
J8668_RS10565 (J8668_10565) 2268920..2269336 + 417 WP_003410114.1 hypothetical protein -
J8668_RS10570 (J8668_10570) 2269352..2269651 - 300 WP_003410120.1 hypothetical protein -
J8668_RS10575 (J8668_10575) 2269736..2270041 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
J8668_RS10580 (J8668_10580) 2270050..2270655 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J8668_RS10585 (J8668_10585) 2270680..2271039 - 360 WP_003410131.1 hypothetical protein -
J8668_RS10590 (J8668_10590) 2271199..2271594 - 396 WP_079156352.1 hypothetical protein -
J8668_RS10595 (J8668_10595) 2271654..2273171 - 1518 Protein_2117 DEAD/DEAH box helicase family protein -
J8668_RS10600 (J8668_10600) 2273681..2274679 - 999 WP_003410146.1 cation diffusion facilitator family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T198661 WP_003900449.1 NZ_CP072765:c2270655-2270050 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T198661 NZ_CP096942:c5885057-5884767 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGATAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT198661 WP_003410124.1 NZ_CP072765:c2270041-2269736 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT198661 NZ_CP096942:c5885353-5885054 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References