Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2204649..2205517 Replicon chromosome
Accession NZ_CP072765
Organism Mycobacterium tuberculosis strain H37Rv_CG

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag J8668_RS10205 Protein ID WP_010886136.1
Coordinates 2204649..2205026 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag J8668_RS10210 Protein ID WP_003409886.1
Coordinates 2205068..2205517 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8668_RS10155 (J8668_10155) 2200438..2200890 - 453 WP_003899095.1 lipoprotein -
J8668_RS10160 (J8668_10160) 2200954..2201355 + 402 WP_003409869.1 hypothetical protein -
J8668_RS10165 (J8668_10165) 2201348..2201530 - 183 WP_003409870.1 hypothetical protein -
J8668_RS10170 (J8668_10170) 2201644..2201994 - 351 WP_003899096.1 hypothetical protein -
J8668_RS10175 (J8668_10175) 2202005..2202907 - 903 WP_003899097.1 hypothetical protein -
J8668_RS10180 (J8668_10180) 2202928..2203119 - 192 WP_003409876.1 hypothetical protein -
J8668_RS10185 (J8668_10185) 2203120..2203416 - 297 WP_003409877.1 hypothetical protein -
J8668_RS10190 (J8668_10190) 2203656..2203871 + 216 WP_003409878.1 antitoxin -
J8668_RS10195 (J8668_10195) 2203868..2204179 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
J8668_RS10200 (J8668_10200) 2204153..2204674 - 522 WP_003904745.1 hypothetical protein -
J8668_RS10205 (J8668_10205) 2204649..2205026 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
J8668_RS10210 (J8668_10210) 2205068..2205517 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
J8668_RS10215 (J8668_10215) 2205514..2206059 + 546 WP_003409891.1 SecB-like chaperone -
J8668_RS10220 (J8668_10220) 2205948..2206562 - 615 WP_003901296.1 hypothetical protein -
J8668_RS10225 (J8668_10225) 2206611..2206907 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
J8668_RS10230 (J8668_10230) 2206904..2207155 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
J8668_RS10235 (J8668_10235) 2207142..2207636 + 495 WP_003899099.1 hypothetical protein -
J8668_RS10240 (J8668_10240) 2207796..2208203 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
J8668_RS10245 (J8668_10245) 2208207..2208479 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
J8668_RS10250 (J8668_10250) 2208512..2209732 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T198655 WP_010886136.1 NZ_CP072765:2204649-2205026 [Mycobacterium tuberculosis]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T198655 NZ_CP096942:c2293968-2293390 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTGTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTCCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAGGCCGCGCACCCGGACAAAGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCACCTCGACGC
GCCGCTGCGCGACCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGTCCGCCGAAGCCCTGGCAGTGAAGGCCC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGCGCCTCGC
AGCTCGGCGCGACGCCTAG

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT198655 WP_003409886.1 NZ_CP072765:2205068-2205517 [Mycobacterium tuberculosis]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT198655 NZ_CP096942:c2294611-2294057 [Pseudomonas aeruginosa]
ATGACCGAGCCTCTCCCTTCTTCGTCCCTGGGCCCCGCCCTGCGTCGCTGGCGCCTGCTGCACCGGGTCAAGCAAACGCA
TGCCGCCGAATTGCTCGGCGTGGCGCAATCGACCATCTCGCGCTGGGAGTCCGGTACCCAGGCGCTGCTGGTGGAGGAAC
GGGCACGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAGCCGCCGCCGACCAGGCACTGGCCCGCCTGGTCGAGGCCAAC
CCGCAACCCGTGCACCTGATCTGCGACCTCACCCATCGCCTGTTGGCCTGTTCGCCGGCACGCGCCGCGCAATTCGGCGT
GCCTCTCGGCGAACTGCTCGACCGTTCGCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAGCCACCCTCGAGG
AACTCGGCTGGCGCGAACTGCTGGCACCGCCGGCGCTGGAGTTCGCCAGCGGAGCGAACGCCTCGGCCATCGTGCCGATC
CGGCGCAGCCGTTGCCGCTGGACCCGCATGACCCTCTCGGACGGACGCGCGGTGCGCCTGGTGGAAACCCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References