Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2981582..2982230 Replicon chromosome
Accession NZ_CP072764
Organism Mycobacterium tuberculosis strain CG20

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag J8671_RS13950 Protein ID WP_003899414.1
Coordinates 2981582..2981905 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag J8671_RS13955 Protein ID WP_003899415.1
Coordinates 2981985..2982230 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8671_RS13930 (J8671_13930) 2977156..2978417 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
J8671_RS13935 (J8671_13935) 2978791..2980230 - 1440 WP_009935349.1 phage major capsid protein -
J8671_RS13940 (J8671_13940) 2980238..2980771 - 534 WP_003899412.1 HK97 family phage prohead protease -
J8671_RS13945 (J8671_13945) 2980924..2981415 - 492 WP_003900541.1 phage terminase small subunit P27 family -
J8671_RS13950 (J8671_13950) 2981582..2981905 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
J8671_RS13955 (J8671_13955) 2981985..2982230 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
J8671_RS13960 (J8671_13960) 2982227..2983654 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
J8671_RS13965 (J8671_13965) 2983656..2984048 - 393 WP_003899417.1 DUF2742 domain-containing protein -
J8671_RS13970 (J8671_13970) 2984045..2984305 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
J8671_RS13975 (J8671_13975) 2984322..2984684 - 363 WP_003900543.1 hypothetical protein -
J8671_RS13980 (J8671_13980) 2984687..2985814 - 1128 WP_003899420.1 site-specific integrase -
J8671_RS13985 (J8671_13985) 2985959..2986186 - 228 WP_003899421.1 hypothetical protein -
J8671_RS13990 (J8671_13990) 2986183..2986572 - 390 WP_003899422.1 hypothetical protein -
J8671_RS13995 (J8671_13995) 2986478..2986750 + 273 WP_003900544.1 hypothetical protein -
J8671_RS14000 (J8671_14000) 2986849..2987082 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2975547..2985814 10267


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-138)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(33-91)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T198610 WP_003899414.1 NZ_CP072764:c2981905-2981582 [Mycobacterium tuberculosis]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T198610 NZ_CP096920:c4010714-4010277 [Pseudomonas putida]
GTGATTTTGTGGCGAATCAGCGCCTATGCAGACTTGAGTGGTACGGGGGGCTTGCGTGTAAGTGGCCGCTGGCACCAGGC
GGGGCGGCCGGTGGTGTATGCCGCCACCAGCCCTCCCGGGGCGATGCTGGAAGTGTTGGTGCACCTGGAGATCGACCCCG
AGGATTTCCCGACCATCATGCGCTTGCTTCGCATCGAACTGCCCGACACGGTTTCCCAGGCGCAGTTGCCCGCTTTGCAG
CCTGGCTGGTCTGCTCAGCCTGAGTTGACCCGTACGCTGGGCAATCGCTTTCTGGATGACGGTTCGGCATTGCTGCTGCC
GGTGCCAAGCGCGATCATGCCCAGCACCACCAATTACCTGTTCAACCCACGGCACCCGCAGGCGCAGAGCGCGAAAATCC
AGGTTGAGGATTTCACCCCGGACAGCCGCCTGTTCTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT198610 WP_003899415.1 NZ_CP072764:c2982230-2981985 [Mycobacterium tuberculosis]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT198610 NZ_CP096920:c4011160-4010711 [Pseudomonas putida]
ATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCTTGCAGGCGCTGCTGGACATTCCCGAGCT
TGCCAGTGACTTCGAGATTCACACCCGCATCACCGATGGTTTTGCCGCCACCTGGTTGGTAAAGCTGACCGAACGCGGTG
TGCTCACGCCGGTGGAGCGCGACCAGATCATCCCGCTGCGCACCCTCAAATCCCGTATCGAGCGCGACCAACCGCTGACC
GTGGATGAGAGCGATCGGCTGTTCCGTTCGGCCCACATCACCGCCATGGCCGAGGCCGTGTTTGGTGAAGCGGGCAAGGC
CAAACGCTGGCTTTCCAAGCCCAAGGAGCGTTTCTCGGGGCTGACGCCGATGCAGATGCTCACCACCCAGCAAGGCACCA
CGCAGGTCGAAGAAATGCTGCTGCAGATTGCCGAGGGCTATGGCCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References