Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2269735..2270654 Replicon chromosome
Accession NZ_CP072764
Organism Mycobacterium tuberculosis strain CG20

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag J8671_RS10590 Protein ID WP_003900449.1
Coordinates 2270049..2270654 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag J8671_RS10585 Protein ID WP_003410124.1
Coordinates 2269735..2270040 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8671_RS10555 (J8671_10555) 2264746..2266002 - 1257 WP_003410095.1 HNH endonuclease signature motif containing protein -
J8671_RS10560 (J8671_10560) 2266356..2266931 + 576 WP_003410100.1 hypothetical protein -
J8671_RS10565 (J8671_10565) 2266928..2267968 + 1041 WP_003911735.1 ImmA/IrrE family metallo-endopeptidase -
J8671_RS10570 (J8671_10570) 2268210..2268929 + 720 WP_003410108.1 DUF433 domain-containing protein -
J8671_RS10575 (J8671_10575) 2268919..2269335 + 417 WP_003410114.1 hypothetical protein -
J8671_RS10580 (J8671_10580) 2269351..2269650 - 300 WP_003410120.1 hypothetical protein -
J8671_RS10585 (J8671_10585) 2269735..2270040 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
J8671_RS10590 (J8671_10590) 2270049..2270654 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J8671_RS10595 (J8671_10595) 2270679..2271038 - 360 WP_003410131.1 hypothetical protein -
J8671_RS10600 (J8671_10600) 2271198..2271593 - 396 WP_079156352.1 hypothetical protein -
J8671_RS10605 (J8671_10605) 2271653..2273170 - 1518 Protein_2119 DEAD/DEAH box helicase family protein -
J8671_RS10610 (J8671_10610) 2273680..2274678 - 999 WP_003410146.1 cation diffusion facilitator family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T198600 WP_003900449.1 NZ_CP072764:c2270654-2270049 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T198600 NZ_CP096915:c54118-53768 [Pseudomonas aeruginosa]
ATGAGCAAACGCCTGCAGCATCAGTTCGACCGCGGCGATATCGTCTCCTTGGACATGGGTAGCGCTTCAGCACAGGCGGC
CTGCAAAGCGCTCGTGCTTTCCCCTGCAGCGTACAACGTCCTGGGGCTCGCCCTAGCCATTCCTATCACCGAGCACGACA
GCAGCCGCTACGCCGGCTTTGCCGTCGCGATCCTCCTTCCAGGGCGCACGCCGGCGAAGGGGGCAGCCTTGGTCAACCTG
GTGCGGCCGGTGGATCTTGCTGCAAGAGGTGCAAAGCTGCTCGGGAAGGCGCCCCAGACGACCATAGATGAAGCCCTGCA
GCGCCTGCAGGCGGTTGTTGGGAAAGACTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT198600 WP_003410124.1 NZ_CP072764:c2270040-2269735 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT198600 NZ_CP096915:c54363-54115 [Pseudomonas aeruginosa]
TTGAAAACGACTATCCGTGCGATTGGGAACTCTCAGGGCATCACGATACCCAAGCCGTTGCTGGCCGAGCTCGGCGTAAG
CCTTGGCGACCCTGTAGACATCTCCTTCAAAGACCAGACCTTGGTGGTGACCAAGGTCGCCACCGAATATGACCTCAGCA
CGCTAGTCGCCCAGTGTGACCTGGAGGCCCCGGAGCCGCCGGACATCCAATGCTGGAGAACAGCTACGCCGGTAGGAAAC
GAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References