Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2204648..2205516 Replicon chromosome
Accession NZ_CP072764
Organism Mycobacterium tuberculosis strain CG20

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag J8671_RS10215 Protein ID WP_010886136.1
Coordinates 2204648..2205025 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag J8671_RS10220 Protein ID WP_003409886.1
Coordinates 2205067..2205516 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8671_RS10165 (J8671_10165) 2200437..2200889 - 453 WP_003899095.1 lipoprotein -
J8671_RS10170 (J8671_10170) 2200953..2201354 + 402 WP_003409869.1 hypothetical protein -
J8671_RS10175 (J8671_10175) 2201347..2201529 - 183 WP_003409870.1 hypothetical protein -
J8671_RS10180 (J8671_10180) 2201643..2201993 - 351 WP_003899096.1 hypothetical protein -
J8671_RS10185 (J8671_10185) 2202004..2202906 - 903 WP_003899097.1 hypothetical protein -
J8671_RS10190 (J8671_10190) 2202927..2203118 - 192 WP_003409876.1 hypothetical protein -
J8671_RS10195 (J8671_10195) 2203119..2203415 - 297 WP_003409877.1 hypothetical protein -
J8671_RS10200 (J8671_10200) 2203655..2203870 + 216 WP_003409878.1 antitoxin -
J8671_RS10205 (J8671_10205) 2203867..2204178 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
J8671_RS10210 (J8671_10210) 2204152..2204673 - 522 WP_003904745.1 hypothetical protein -
J8671_RS10215 (J8671_10215) 2204648..2205025 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
J8671_RS10220 (J8671_10220) 2205067..2205516 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
J8671_RS10225 (J8671_10225) 2205513..2206058 + 546 WP_003409891.1 SecB-like chaperone -
J8671_RS10230 (J8671_10230) 2205947..2206561 - 615 WP_003901296.1 hypothetical protein -
J8671_RS10235 (J8671_10235) 2206610..2206906 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
J8671_RS10240 (J8671_10240) 2206903..2207154 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
J8671_RS10245 (J8671_10245) 2207141..2207635 + 495 WP_003899099.1 hypothetical protein -
J8671_RS10250 (J8671_10250) 2207795..2208202 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
J8671_RS10255 (J8671_10255) 2208206..2208478 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
J8671_RS10260 (J8671_10260) 2208511..2209731 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T198594 WP_010886136.1 NZ_CP072764:2204648-2205025 [Mycobacterium tuberculosis]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T198594 NZ_CP096913:3824439-3825017 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTGTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTTCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAAGCCGCGCACCCGGACAAGGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCATCTGGACAC
GCCGCTGCGCGATCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGACCGCCGAAGCCCTGGCAGTGAAAGCGC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGTTACTCGACGCCAGGACCCGACAGCGCCTCGC
AGCTCGGCGCGACGCCTAG

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT198594 WP_003409886.1 NZ_CP072764:2205067-2205516 [Mycobacterium tuberculosis]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT198594 NZ_CP096913:3823797-3824351 [Pseudomonas aeruginosa]
ATGACCGAGCCTCTCCCTTCTTCGTCCCTGGGCCCCGCCCTGCGTCGCTGGCGCCTGCTGCACCGGGTCAAGCAAACGCA
TGCCGCCGAATTGCTCGGCGTGGCGCAATCGACCATCTCGCGCTGGGAGTCCGGTACCCAGGCGCTGCTGGTGGAGGAAC
GGGCACGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAGCCGCCGCCGACCAGGCACTGGCCCGCCTGGTCGAGGCCAAC
CCGCAACCCGTGCACCTGATCTGCGACCTCACCCATCGCTTGCTGGCCTGTTCGCCGGCACGCGCCGCGCAATTCGGCGT
GCCCCTCGGCGAACTGCTCGACCGTTCGCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAGCCACCCTCGAGG
AACTCGGCTGGCGCGAACTGCTGGCACCGCCGGCGCTGGAGTTCGCCAGCGGAGCGAACGCCTCGGCCATCGTGCCGATC
CGGCGCAGCCGTTGCCGCTGGACCCGCATGACCCTCTCGGACGGACGCGCGGTGCGCCTGGTGGAAACCCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References