Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3555359..3556029 Replicon chromosome
Accession NZ_CP072763
Organism Mycobacterium tuberculosis strain CG21

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag J8666_RS16715 Protein ID WP_003899954.1
Coordinates 3555359..3555703 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag J8666_RS16720 Protein ID WP_003899955.1
Coordinates 3555700..3556029 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8666_RS16685 (J8666_16685) 3550432..3551292 + 861 WP_003416628.1 alpha/beta hydrolase -
J8666_RS16690 (J8666_16690) 3551267..3551782 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
J8666_RS20935 3551798..3552009 + 212 Protein_3333 (R)-hydratase -
J8666_RS16695 (J8666_16695) 3552022..3552315 + 294 WP_003416635.1 hypothetical protein -
J8666_RS16700 (J8666_16700) 3552603..3553892 + 1290 WP_003416640.1 ATP-binding protein -
J8666_RS16705 (J8666_16705) 3554239..3554673 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
J8666_RS16710 (J8666_16710) 3554676..3555128 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
J8666_RS16715 (J8666_16715) 3555359..3555703 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J8666_RS16720 (J8666_16720) 3555700..3556029 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
J8666_RS16725 (J8666_16725) 3556266..3557527 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
J8666_RS16730 (J8666_16730) 3557749..3559010 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
J8666_RS16735 (J8666_16735) 3559283..3559630 + 348 WP_003899956.1 DUF2384 domain-containing protein -
J8666_RS16740 (J8666_16740) 3559627..3560247 + 621 WP_003899957.1 RES family NAD+ phosphorylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T198557 WP_003899954.1 NZ_CP072763:3555359-3555703 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T198557 NZ_CP096902:2777029-2777136 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT198557 WP_003899955.1 NZ_CP072763:3555700-3556029 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT198557 NZ_CP096902:c2776982-2776916 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References