Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3571873..3572543 Replicon chromosome
Accession NZ_CP072762
Organism Mycobacterium tuberculosis strain CG23

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag J8672_RS16885 Protein ID WP_003899954.1
Coordinates 3571873..3572217 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag J8672_RS16890 Protein ID WP_003899955.1
Coordinates 3572214..3572543 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8672_RS16855 (J8672_16855) 3566946..3567806 + 861 WP_003416628.1 alpha/beta hydrolase -
J8672_RS16860 (J8672_16860) 3567781..3568296 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
J8672_RS21105 3568312..3568523 + 212 Protein_3368 (R)-hydratase -
J8672_RS16865 (J8672_16865) 3568536..3568829 + 294 WP_003416635.1 hypothetical protein -
J8672_RS16870 (J8672_16870) 3569117..3570406 + 1290 WP_003416640.1 ATP-binding protein -
J8672_RS16875 (J8672_16875) 3570753..3571187 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
J8672_RS16880 (J8672_16880) 3571190..3571642 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
J8672_RS16885 (J8672_16885) 3571873..3572217 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J8672_RS16890 (J8672_16890) 3572214..3572543 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
J8672_RS16895 (J8672_16895) 3572780..3574041 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
J8672_RS16900 (J8672_16900) 3574263..3575524 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
J8672_RS16905 (J8672_16905) 3575797..3576144 + 348 WP_003899956.1 DUF2384 domain-containing protein -
J8672_RS16910 (J8672_16910) 3576141..3576761 + 621 WP_003899957.1 RES family NAD+ phosphorylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T198496 WP_003899954.1 NZ_CP072762:3571873-3572217 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T198496 NZ_CP096848:1169813-1170694 [Mycobacterium tuberculosis variant bovis]
GTGACCAAGCCCTATTCGTCGCCGCCAACGAACCTGCGCTCACTACGAGATCGGCTCACCCAAGTAGCGGAACGGCAAGG
TGTCGTGTTCGGTCGACTGCAGCGGCATGTCGCGATGATTGTTGTCGCACAGTTCGCGGCCACGCTCACCGACGACACCG
GCGCTCCGCTGCTGTTGGTCAAAGGCGGATCGTCGCTGGAACTGCGCCGGGGAATTCCCGATTCGCGGACCTCCAAAGAC
TTCGACACGGTCGCACGTCGCGATATCGAATTAATCCATGAACAGCTCGCTGACGCGGGCGAGACGGGGTGGGAAGGATT
CACTGCAATCTTCACCGCCCCCGAAGAAATCGATGTTCCTGGTATGCCGGTCAAGCCGCGCCGATTCACCGCCAAGCTGA
GCTACCGAGGCCGGGCTTTCGCAACTGTTCCGATCGAGGTCTCCTCCGTCGAAGCCGGCAATGCCGACCAATTCGACACC
CTCACCTCAGACGCGCTCGGCCTCGTGGGCGTACCCGCAGCAGTCGCCGTACCCTGCATGACCATTCCCTGGCAAATCGC
GCAGAAGCTGCACGCAGTAACTGCCGTGCTCGAAGAACCGAAGGTCAACGACCGCGCTCACGACCTGGTGGACTTGCAGC
TTCTTGAAGGACTGTTGCTCGATGCCGACCTCATGCCGACGCGCAGCGCGTGCATCGCGATATTCGAAGCGCGCGCCCAG
CATCCTTGGCCACCGAGAGTCGCCACGCTGCCGCACTGGCCGCTGATCTATGCAGGTGCGCTGGAGGGGCTTGACCACCT
TGAACTCGCCAGGACGGTCGACGCGGCGGCCCAGGCAGTGCAGCGATTCGTTGCGCGGATTGATCGGGCGACGAAAAGAT
GA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT198496 WP_003899955.1 NZ_CP072762:3572214-3572543 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT198496 NZ_CP096848:1169193-1169816 [Mycobacterium tuberculosis variant bovis]
TTGTGTGCAAAACCGTATCTAATTGATACGATTGCGCACATGGCTATCTGGGATCGCCTCGTCGAGGTTGCCGCCGAGCA
ACATGGCTACGTCACGACTCGCGATGCGCGAGACATCGGCGTCGACCCTGTGCAGCTCCGCCTCCTAGCGGGGCGCGGAC
GTCTTGAGCGTGTCGGCCGAGGTGTGTACCGGGTGCCCGTGCTGCCGCGTGGTGAGCACGACGATCTCGCAGCCGCAGTG
TCGTGGACTTTGGGGCGTGGCGTTATCTCGCATGAGTCGGCCTTGGCGCTTCATGCCCTCGCTGACGTGAACCCGTCGCG
CATCCATCTCACCGTCCCGCGCAACAACCATCCGCGTGCGGCCGGGGGCGAGCTGTACCGAGTTCACCGCCGCGACCTCC
AGGCAGCCCACGTCACTTCGGTCGACGGAATACCCGTCACGACGGTTGCGCGCACCATCAAAGACTGCGTGAAGACGGGC
ACGGATCCTTATCAGCTTCGGGCCGCGATCGAGCGAGCCGAAGCCGAGGGCACGCTTCGTCGTGGGTCAGCAGCTGAGCT
ACGCGCTGCGCTCGATGAGACCACTGCCGGATTACGCGCTCGGCCGAAGCGAGCATCGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References