Detailed information of TA system TA198478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2269736..2270655
• Replicon: chromosome
• Accession: NZ_CP072762
• Organism: Mycobacterium tuberculosis strain CG23

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: L7N4R2
• Locus tag: J8672_RS10580
• Protein ID: WP_003900449.1
• Coordinates: 2270050..2270655 (-)
• Length: 202 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: L7N5K9
• Locus tag: J8672_RS10575
• Protein ID: WP_003410124.1
• Coordinates: 2269736..2270041 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8672_RS10545 (J8672_10545)	2264747..2266003	-	1257	WP_003410095.1	HNH endonuclease signature motif containing protein	-
J8672_RS10550 (J8672_10550)	2266357..2266932	+	576	WP_003410100.1	hypothetical protein	-
J8672_RS10555 (J8672_10555)	2266929..2267969	+	1041	WP_003911735.1	ImmA/IrrE family metallo-endopeptidase	-
J8672_RS10560 (J8672_10560)	2268211..2268930	+	720	WP_003410108.1	DUF433 domain-containing protein	-
J8672_RS10565 (J8672_10565)	2268920..2269336	+	417	WP_003410114.1	hypothetical protein	-
J8672_RS10570 (J8672_10570)	2269352..2269651	-	300	WP_003410120.1	hypothetical protein	-
J8672_RS10575 (J8672_10575)	2269736..2270041	-	306	WP_003410124.1	XRE family transcriptional regulator	Antitoxin
J8672_RS10580 (J8672_10580)	2270050..2270655	-	606	WP_003900449.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J8672_RS10585 (J8672_10585)	2270680..2271039	-	360	WP_003410131.1	hypothetical protein	-
J8672_RS10590 (J8672_10590)	2271199..2271594	-	396	WP_079156352.1	hypothetical protein	-
J8672_RS10595 (J8672_10595)	2271654..2273171	-	1518	Protein_2117	DEAD/DEAH box helicase family protein	-
J8672_RS10600 (J8672_10600)	2273681..2274679	-	999	WP_003410146.1	cation diffusion facilitator family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (86-181)

Antitoxin

• HigA2-like, helix-turn-helix domain (32-92)

Sequences

Toxin

Protein

• Length: 202 a.a.
• Molecular weight: 22744.96 Da
• Isoelectric Point: 7.3073

>T198478 WP_003900449.1 NZ_CP072762:c2270655-2270050 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Nucleotide

• Length: 606 bp

>T198478 NZ_CP096848:71513-71914 [Mycobacterium tuberculosis variant bovis]
GTGGATGAATGTGTAGTCGACGCGGCGGCCGTGGTTGACGCTCTCGCCGGCAAGGGCGCCAGCGCGATCGTTCTGCGCGG
TTTGCTCAAGGAGTCGATTTCTAACGCGCCGCATTTGCTGGACGCAGAGGTCGGACATGCACTCCGCCGCGCCGTGCTCA
GCGACGAAATCTCCGAAGAGCAGGCTCGCGCCGCGTTGGATGCCTTGCCTTATCTCATCGACAATCGTTACCCGCACAGC
CCACGACTGATCGAATACACATGGCAGCTAAGGCACAACGTCACGTTCTACGACGCCCTTTACGTCGCACTGGCCACCGC
ACTGGATGTCCCGCTGCTCACGGGCGACTCGCGGCTTGCGGCCGCGCCGGGCCTTCCGTGCGAAATCAAACTCGTTCGGT
GA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11291.91 Da
• Isoelectric Point: 10.7482

>AT198478 WP_003410124.1 NZ_CP072762:c2270041-2269736 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Nucleotide

• Length: 306 bp

>AT198478 NZ_CP096848:71281-71520 [Mycobacterium tuberculosis variant bovis]
ATGGCTACCATTCAAGTTCGGGATTTGCCCGAAGATGTCGCCGAAACCTATCGACGGCGCGCCACCGCAGCGGGGCAGTC
GCTGCAGACGTATATGCGCACCAAGCTCATCGAAGGGGTGCGGGGCCGAGACAAGGCCGAGGTAATCGAGATCCTGGAAC
AGGCGCTCGCCAGCACTGCCAGCCCAGGCATCAGCCGGGAGACCATCGAGGCATCCCGGCGGGAGCTCAGGGGTGGATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BV20

Antitoxin

• AlphaFold DB: A0A7U4BV30