Detailed information of TA system TA198368

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3782405..3783065
• Replicon: chromosome
• Accession: NZ_CP072742
• Organism: Serratia fonticola strain R28

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: J8M99_RS17245
• Protein ID: WP_218519157.1
• Coordinates: 3782405..3782758 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: U2NFA4
• Locus tag: J8M99_RS17250
• Protein ID: WP_021180280.1
• Coordinates: 3782763..3783065 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8M99_RS17225 (J8M99_17215)	3777915..3779201	+	1287	WP_218519156.1	high-affinity branched-chain amino acid ABC transporter permease LivM	-
J8M99_RS17230 (J8M99_17220)	3779198..3780073	+	876	WP_021180270.1	ATP-binding cassette domain-containing protein	-
J8M99_RS17235 (J8M99_17225)	3780070..3780786	+	717	WP_021805811.1	ABC transporter ATP-binding protein	-
J8M99_RS17240 (J8M99_17230)	3780960..3782147	+	1188	WP_021805810.1	sugar transporter	-
J8M99_RS17245 (J8M99_17235)	3782405..3782758	+	354	WP_218519157.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J8M99_RS17250 (J8M99_17240)	3782763..3783065	+	303	WP_021180280.1	XRE family transcriptional regulator	Antitoxin
J8M99_RS17255 (J8M99_17245)	3783209..3784069	+	861	WP_218519158.1	aldo/keto reductase family oxidoreductase	-
J8M99_RS17260 (J8M99_17250)	3784329..3785531	+	1203	WP_094982866.1	Bcr/CflA family multidrug efflux MFS transporter	-
J8M99_RS17265 (J8M99_17255)	3785604..3786548	-	945	WP_071680978.1	flavin reductase family protein	-
J8M99_RS17270 (J8M99_17260)	3786569..3787738	-	1170	WP_235784772.1	flavin-dependent monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13602.52 Da
• Isoelectric Point: 9.5694

>T198368 WP_218519157.1 NZ_CP072742:3782405-3782758 [Serratia fonticola]
VWEIKTTDTFDEWFSRLEDTDRASVLASLIVLREKGPQLPRPYADTVKGSRYSNMKELRVQSRGIPIRAFFAFDPRRRGI
LLCAGNKAGNEKRFYEVMIPIADREFTQFLNTAENKE

Nucleotide

• Length: 354 bp

>T198368 NZ_CP096846:c445560-444967 [Mycobacterium tuberculosis variant bovis]
GTGAAGCGGCTCGATCTGGTCGCCGGGCCCAACGGCGCCGGCAAGTCGACGTTCGTCGCCCTCACGCTGGCGCCCTTGCT
GCCCGGCATCGTCTTCGTCAACGCCGACGAAATCGCCAAACAACGCTGGCCCGACGACCCAACATCGCACGCCTACCAGG
CGGCGCAGGTCGCCGCCGACACCCGCGCGAGGCTCATCGACTTGGGCCGGCCGTTCATTGCCGAGACGGTGTTCTCGCAC
CCATCGAAGCTCGAGCTCATCCGCACCGCGCGCACGGCCGGCTACACCGTCGTACTGCACGTGTTGGTTATCCCCGAAGG
CCTGGCGGTCGAGCGCGTCAGGCATCGCGTCGCCGCGGGCGGCCACGATGTGCCGGAGACCAAGATCCGCGAGCGTCACC
GCCGGCTGGCCGAGCTTGTCGCCCAGGCCATCACGCTGGCCGACGGCGCCACCGTCTATGACAACAGCCGGCTCGCGGGC
CCGCGGATCGTCGCTCAGTTCAGCGGGGGTGGCATCATCGGGCGCGCATGCTGGCCTTCGTGGACGCCGCCACCCTTGAT
GTCACGCTGGAGTAACAGGCCTGAGACGGCGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11022.78 Da
• Isoelectric Point: 6.4749

>AT198368 WP_021180280.1 NZ_CP072742:3782763-3783065 [Serratia fonticola]
MGRTLEQILAEEKPEVVASAERIAADTLLNIHLAELRERVQKTQVEMAQALGIKQPTVAGMEKPGRDLKLSSLKRYIEAA
GGKLRLDIELPDGSHYGFVI

Nucleotide

• Length: 303 bp

>AT198368 NZ_CP096846:c445978-445589 [Mycobacterium tuberculosis variant bovis]
GTGCCCAAAGCCGTCGACCGCGTCACGCGCGTCGCCGCGGATCTGGTGGACAGCGCGGCGGCCGAGGGTGCTCGTCAGAG
CCGTTCGGCCAAGCAGCAGCTCGACCACTGGGCCCGCGTCGGTCGGGCGGTGTCGAACCAGCACACGGCGTCGCGGCGCC
GCGTGGAAGCTGCGCTCGCGGGGCATCTGCCGATGACGGACCTGACGCTGGAGGAGGGCGTGGTGTTCAACGCCGAGATC
TCCGCAGCTATCGAGGAGCGCCTGTCGCGAACCAACTACGGCGACGTGCTCGCCGCGCAGGGCATCACCACGGTCGCCCT
GAACGACGCGGGCGACATCGTCGAGCACCGGCCAGACGGCACCTCGGTCGTGCTGGCGGCCACGCCGTGA

Structures

Antitoxin

• AlphaFold DB: U2NFA4