Detailed information of TA system TA198363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 1774718..1775370
• Replicon: chromosome
• Accession: NZ_CP072742
• Organism: Serratia fonticola strain R28

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U2LU32
• Locus tag: J8M99_RS08160
• Protein ID: WP_021181419.1
• Coordinates: 1774718..1775059 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J8M99_RS08165
• Protein ID: WP_094982460.1
• Coordinates: 1775056..1775370 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8M99_RS08135 (J8M99_08130)	1769803..1770534	-	732	WP_024484696.1	phosphate signaling complex protein PhoU	-
J8M99_RS08140 (J8M99_08135)	1770547..1771323	-	777	WP_021181414.1	phosphate ABC transporter ATP-binding protein PstB	-
J8M99_RS08145 (J8M99_08140)	1771371..1772261	-	891	WP_059199278.1	phosphate ABC transporter permease PstA	-
J8M99_RS08150 (J8M99_08145)	1772263..1773219	-	957	WP_021181416.1	phosphate ABC transporter permease PstC	-
J8M99_RS08155 (J8M99_08150)	1773310..1774350	-	1041	WP_021181417.1	phosphate ABC transporter substrate-binding protein PstS	-
J8M99_RS08160 (J8M99_08155)	1774718..1775059	+	342	WP_021181419.1	hypothetical protein	Toxin
J8M99_RS08165 (J8M99_08160)	1775056..1775370	+	315	WP_094982460.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
J8M99_RS08170 (J8M99_08165)	1775468..1776547	-	1080	WP_218520081.1	GNAT family N-acetyltransferase	-
J8M99_RS08175 (J8M99_08170)	1776549..1777664	-	1116	WP_218520082.1	hypothetical protein	-
J8M99_RS08180 (J8M99_08175)	1777787..1779616	-	1830	WP_218520083.1	glutamine--fructose-6-phosphate transaminase (isomerizing)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-88)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13473.66 Da
• Isoelectric Point: 9.7468

>T198363 WP_021181419.1 NZ_CP072742:1774718-1775059 [Serratia fonticola]
MDAIFVELSIFEAKRADYLSDDEFKTFQQMLLINPFCGDVIQHTGGLRKVRFADHRRGKGKRGGLRVIYYWWLEKSHFLL
FVIYDKDKKDDLSRNEREMLRRMLEILKQGGTL

Nucleotide

• Length: 342 bp

>T198363 NZ_CP096845:3771952-3772362 [Mycobacterium tuberculosis variant bovis]
GTGATCTATATGGACACCTCGGCCCTGACTAAGCTGCTCATCTCCGAGCCCGAGACGACCGAACTGCGGACATGGCTGAC
CGCGCAAAGCGGCCAGGGCGAGGACGCGGCGACAAGCACCCTTGGCCGGGTCGAGTTGATGAGAGTCGTTGCCCGATACG
GACAACCAGGCCAAACTGAGCGTGCGCGTTACCTACTCGACGGGCTCGACATCCTCCCGCTCACCGAACCGGTGATCGGT
CTAGCTGAAACGATCGGACCGGCCACCCTACGTTCTCTCGACGCGATTCACCTCGCGGCCGCAGCCCAGATCAAGCGGGA
ACTGACAGCCTTCGTCACCTACGACCACCGATTGTTGAGCGGATGCCGTGAGGTCGGCTTCGTCACCGCCTCACCCGGCG
CAGTCCGGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11884.72 Da
• Isoelectric Point: 7.2018

>AT198363 WP_094982460.1 NZ_CP072742:1775056-1775370 [Serratia fonticola]
MSELSLFEELSQGMKDWSDYNEGKITLKTHAISLKKNISMSPDELKAIREKLRLSQAVFADYLHTGVTTYQNWEQGRAKP
NKQALLLIRMVEKDPMTLDTLAAL

Nucleotide

• Length: 315 bp

>AT198363 NZ_CP096845:3771677-3771955 [Mycobacterium tuberculosis variant bovis]
GTGGAGGCAATCGGAATCCGAGAACTAAGACAGCACGCATCGCGATACCTCGCCCGGGTTGAAGCCGGCGAGGAACTTGG
CGTCACCAACAAAGGAAGACTTGTGGCCCGACTCATCCCGGTGCAGGCCGCGGAGCGTTCTCGCGAAGCCCTGATTGAAT
CAGGTGTCCTGATTCCGGCTCGTCGTCCACAAAACCTTCTCGACGTCACCGCCGAACCGGCGCGCGGCCGCAAGCGCACC
CTGTCCGATGTTCTCAACGAAATGCGCGACGAGCAGTGA

Structures

Toxin

• AlphaFold DB: U2LU32