Detailed information of TA system TA198343

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 213784..214319
• Replicon: plasmid pVB3378_p1
• Accession: NZ_CP072708
• Organism: Enterococcus faecium strain VB3378

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: R2PQC0
• Locus tag: J7155_RS14655
• Protein ID: WP_002314366.1
• Coordinates: 213784..214056 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: Q3Y220
• Locus tag: J7155_RS14660
• Protein ID: WP_002297422.1
• Coordinates: 214056..214319 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7155_RS14615 (J7155_14590)	208891..209340	-	450	WP_010729386.1	TlpA disulfide reductase family protein	-
J7155_RS14620 (J7155_14595)	209496..210038	-	543	WP_002298088.1	hypothetical protein	-
J7155_RS14625 (J7155_14600)	210313..210956	+	644	Protein_230	transposase	-
J7155_RS14630 (J7155_14605)	211032..211712	+	681	WP_002331383.1	IS6-like element IS1216 family transposase	-
J7155_RS14635 (J7155_14610)	211771..211959	-	189	Protein_232	hypothetical protein	-
J7155_RS14640 (J7155_14615)	211960..212817	-	858	WP_002292680.1	ParA family protein	-
J7155_RS14645 (J7155_14620)	213102..213388	-	287	Protein_234	type II toxin-antitoxin system RelE/ParE family toxin	-
J7155_RS14650 (J7155_14625)	213378..213707	-	330	WP_002292678.1	AbrB family transcriptional regulator	-
J7155_RS14655 (J7155_14630)	213784..214056	-	273	WP_002314366.1	Txe/YoeB family addiction module toxin	Toxin
J7155_RS14660 (J7155_14635)	214056..214319	-	264	WP_002297422.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
J7155_RS14665 (J7155_14640)	214553..215461	+	909	Protein_238	IS982-like element ISEfm1 family transposase	-
J7155_RS14670 (J7155_14645)	215464..216639	-	1176	WP_002336205.1	MFS transporter	-
J7155_RS14675 (J7155_14650)	216642..218891	-	2250	WP_002314387.1	alpha-galactosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-aph(2'')	-	1..227172	227172
	-	inside	IScluster/Tn	-	-	202222..224920	22698

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (9-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-69)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10780.24 Da
• Isoelectric Point: 9.9724

>T198343 WP_002314366.1 NZ_CP072708:c214056-213784 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Nucleotide

• Length: 273 bp

>T198343 NZ_CP096845:2232687-2233085 [Mycobacterium tuberculosis variant bovis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9959.22 Da
• Isoelectric Point: 4.5370

>AT198343 WP_002297422.1 NZ_CP072708:c214319-214056 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Nucleotide

• Length: 264 bp

>AT198343 NZ_CP096845:2232459-2232686 [Mycobacterium tuberculosis variant bovis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCGACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• AlphaFold DB: A0A829F704

Antitoxin

• AlphaFold DB: A0A829F006