Detailed information of TA system TA198325

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2384258..2384629
• Replicon: chromosome
• Accession: NZ_CP072689
• Organism: Escherichia marmotae strain YF8

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A1Y2ZGM7
• Locus tag: J8I93_RS11215
• Protein ID: WP_042038331.1
• Coordinates: 2384258..2384452 (+)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2384451..2384629 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8I93_RS11190 (2379956)	2379956..2380177	+	222	WP_000560223.1	killing protein KilR	-
J8I93_RS11195 (2380177)	2380177..2380347	+	171	WP_001485242.1	YdaE family protein	-
J8I93_RS11200 (2380422)	2380422..2380697	+	276	WP_000632297.1	protein RacC	-
J8I93_RS11205 (2380799)	2380799..2383399	+	2601	WP_024008686.1	exodeoxyribonuclease VIII	-
J8I93_RS11210 (2383392)	2383392..2384201	+	810	WP_000166319.1	recombination protein RecT	-
J8I93_RS11215 (2384258)	2384258..2384452	+	195	WP_042038331.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
- (2384451)	2384451..2384629	-	179	NuclAT_0	-	Antitoxin
- (2384451)	2384451..2384629	-	179	NuclAT_0	-	Antitoxin
- (2384451)	2384451..2384629	-	179	NuclAT_0	-	Antitoxin
- (2384451)	2384451..2384629	-	179	NuclAT_0	-	Antitoxin
J8I93_RS11220 (2384445)	2384445..2384633	+	189	WP_001302840.1	DUF1187 family protein	-
J8I93_RS11225 (2384733)	2384733..2384948	+	216	WP_000079604.1	excisionase XisR	-
J8I93_RS11230 (2384950)	2384950..2386185	+	1236	WP_024008685.1	site-specific integrase	-
J8I93_RS11235 (2386237)	2386237..2387172	+	936	WP_016242657.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
J8I93_RS11240 (2387267)	2387267..2388640	-	1374	WP_038354512.1	ATP-dependent RNA helicase DbpA	-
J8I93_RS11245 (2388670)	2388670..2388843	-	174	WP_001296046.1	protein YnaL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2338989..2386185	47196

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.94 Da
• Isoelectric Point: 8.9538

>T198325 WP_042038331.1 NZ_CP072689:2384258-2384452 [Escherichia marmotae]
MRYDNVKPCPFCGCPSVTVKVISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T198325 NZ_CP096845:1012183-1012617 [Mycobacterium tuberculosis variant bovis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT198325 NZ_CP072689:c2384629-2384451 [Escherichia marmotae]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A1Y2ZGM7

Antitoxin