Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2633459..2634021 Replicon chromosome
Accession NZ_CP072688
Organism Escherichia coli strain P4

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag J8I92_RS12830 Protein ID WP_000605675.1
Coordinates 2633459..2633737 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag J8I92_RS12835 Protein ID WP_000781370.1
Coordinates 2633737..2634021 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8I92_RS12805 (2629047) 2629047..2629478 - 432 WP_000152305.1 peroxiredoxin OsmC -
J8I92_RS12810 (2629823) 2629823..2630038 + 216 WP_000495766.1 biofilm-dependent modulation protein -
J8I92_RS12815 (2630140) 2630140..2630277 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
J8I92_RS12820 (2630433) 2630433..2632130 + 1698 WP_000433462.1 malate dehydrogenase -
J8I92_RS12825 (2632263) 2632263..2633273 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
J8I92_RS12830 (2633459) 2633459..2633737 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J8I92_RS12835 (2633737) 2633737..2634021 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
J8I92_RS12840 (2634250) 2634250..2634903 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
J8I92_RS12845 (2634896) 2634896..2635780 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
J8I92_RS12850 (2635793) 2635793..2638840 - 3048 WP_077634457.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T198303 WP_000605675.1 NZ_CP072688:2633459-2633737 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T198303 NZ_CP096844:3152062-3152505 [Mycobacterium tuberculosis variant bovis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT198303 WP_000781370.1 NZ_CP072688:2633737-2634021 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT198303 NZ_CP096844:3151818-3152075 [Mycobacterium tuberculosis variant bovis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References