Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1956255..1956757 Replicon chromosome
Accession NZ_CP072688
Organism Escherichia coli strain P4

Toxin (Protein)


Gene name yoeB Uniprot ID D8A584
Locus tag J8I92_RS09420 Protein ID WP_000767828.1
Coordinates 1956503..1956757 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag J8I92_RS09415 Protein ID WP_001259255.1
Coordinates 1956255..1956506 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8I92_RS09390 (1951433) 1951433..1952500 - 1068 WP_000080129.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
J8I92_RS09395 (1952500) 1952500..1953570 - 1071 WP_000108949.1 histidinol-phosphate transaminase -
J8I92_RS09400 (1953567) 1953567..1954871 - 1305 WP_001370829.1 histidinol dehydrogenase -
J8I92_RS09405 (1954877) 1954877..1955776 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
J8I92_RS09410 (1955922) 1955922..1955972 - 51 WP_001364200.1 his operon leader peptide -
J8I92_RS09415 (1956255) 1956255..1956506 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
J8I92_RS09420 (1956503) 1956503..1956757 + 255 WP_000767828.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
J8I92_RS09425 (1956840) 1956840..1957664 + 825 WP_282478837.1 SDR family oxidoreductase -
J8I92_RS09430 (1957710) 1957710..1958639 + 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
J8I92_RS09435 (1958854) 1958854..1958916 + 63 WP_010723108.1 membrane protein YoeI -
J8I92_RS09440 (1958906) 1958906..1960264 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
J8I92_RS09445 (1960443) 1960443..1961501 + 1059 WP_000492324.1 thiosulfate utilization transporter TsuA/YeeE -
J8I92_RS09450 (1961515) 1961515..1961742 + 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10169.56 Da        Isoelectric Point: 8.0353

>T198288 WP_000767828.1 NZ_CP072688:1956503-1956757 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEDHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T198288 NZ_CP096844:2315354-2315764 [Mycobacterium tuberculosis variant bovis]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCGCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT198288 WP_001259255.1 NZ_CP072688:1956255-1956506 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT198288 NZ_CP096844:2315128-2315361 [Mycobacterium tuberculosis variant bovis]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G465

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References