Detailed information of TA system TA198287

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1182830..1183557
• Replicon: chromosome
• Accession: NZ_CP072688
• Organism: Escherichia coli strain P4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: J8I92_RS05725
• Protein ID: WP_000547563.1
• Coordinates: 1182830..1183141 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J8I92_RS05730
• Protein ID: WP_000126304.1
• Coordinates: 1183138..1183557 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8I92_RS05695 (1177974)	1177974..1179683	+	1710	WP_001288123.1	formate hydrogenlyase subunit HycE	-
J8I92_RS05700 (1179693)	1179693..1180235	+	543	WP_000493800.1	formate hydrogenlyase subunit HycF	-
J8I92_RS05705 (1180235)	1180235..1181002	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
J8I92_RS05710 (1180999)	1180999..1181409	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
J8I92_RS05715 (1181402)	1181402..1181869	+	468	WP_000132955.1	hydrogenase maturation peptidase HycI	-
J8I92_RS05720 (1181912)	1181912..1182667	+	756	WP_001314074.1	hypothetical protein	-
J8I92_RS05725 (1182830)	1182830..1183141	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
J8I92_RS05730 (1183138)	1183138..1183557	+	420	WP_000126304.1	helix-turn-helix domain-containing protein	Antitoxin
J8I92_RS05735 (1183649)	1183649..1184077	-	429	WP_000536066.1	DUF4259 domain-containing protein	-
J8I92_RS05740 (1184462)	1184462..1184989	+	528	WP_001078777.1	electron transport protein HydN	-
J8I92_RS05745 (1185142)	1185142..1187394	+	2253	WP_001535518.1	carbamoyltransferase HypF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T198287 WP_000547563.1 NZ_CP072688:1182830-1183141 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T198287 NZ_CP096844:2247570-2247968 [Mycobacterium tuberculosis variant bovis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15474.50 Da
• Isoelectric Point: 4.4596

>AT198287 WP_000126304.1 NZ_CP072688:1183138-1183557 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT198287 NZ_CP096844:2247342-2247569 [Mycobacterium tuberculosis variant bovis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCGACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• AlphaFold DB: A0A7U9QA22