Detailed information of TA system TA198263

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1227461..1228188
• Replicon: chromosome
• Accession: NZ_CP072687
• Organism: Escherichia coli strain YP3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: J8I95_RS05955
• Protein ID: WP_000547563.1
• Coordinates: 1227461..1227772 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J8I95_RS05960
• Protein ID: WP_000126304.1
• Coordinates: 1227769..1228188 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8I95_RS05925 (1222605)	1222605..1224314	+	1710	WP_001288123.1	formate hydrogenlyase subunit HycE	-
J8I95_RS05930 (1224324)	1224324..1224866	+	543	WP_000493800.1	formate hydrogenlyase subunit HycF	-
J8I95_RS05935 (1224866)	1224866..1225633	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
J8I95_RS05940 (1225630)	1225630..1226040	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
J8I95_RS05945 (1226033)	1226033..1226500	+	468	WP_000132955.1	hydrogenase maturation peptidase HycI	-
J8I95_RS05950 (1226543)	1226543..1227298	+	756	WP_001314074.1	hypothetical protein	-
J8I95_RS05955 (1227461)	1227461..1227772	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
J8I95_RS05960 (1227769)	1227769..1228188	+	420	WP_000126304.1	helix-turn-helix domain-containing protein	Antitoxin
J8I95_RS05965 (1228280)	1228280..1228708	-	429	WP_000536066.1	DUF4259 domain-containing protein	-
J8I95_RS05970 (1229093)	1229093..1229620	+	528	WP_001078777.1	electron transport protein HydN	-
J8I95_RS05975 (1229773)	1229773..1232025	+	2253	WP_001467844.1	carbamoyltransferase HypF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1198614..1237533	38919

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T198263 WP_000547563.1 NZ_CP072687:1227461-1227772 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T198263 NZ_CP096844:719164-719571 [Mycobacterium tuberculosis variant bovis]
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15474.50 Da
• Isoelectric Point: 4.4596

>AT198263 WP_000126304.1 NZ_CP072687:1227769-1228188 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT198263 NZ_CP096844:718907-719167 [Mycobacterium tuberculosis variant bovis]
ATGTCTGAGGTGGCCTCGCGTGAGCTGCGTAACGATACGGCCGGCGTGCTGCGCCGCGTGCGGGCAGGGGAGGACGTCAC
CATCACCGTCAGCGGCCGTCCGGTCGCGGTGCTTACCCCGGTTCGTCCGCGGCGCCGGCGTTGGCTGAGCAAAACGGAGT
TCCTGTCGCGGTTGCGCGGCGCTCAAGCCGATCCCGGGCTCCGTAACGACCTCGCGGTCCTTGCCGGCGACACGACCGAG
GATCTCGGGCCGATCCGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22