Detailed information of TA system TA198237

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1182827..1183554
• Replicon: chromosome
• Accession: NZ_CP072686
• Organism: Escherichia coli strain FP4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: J8I94_RS05725
• Protein ID: WP_000547563.1
• Coordinates: 1182827..1183138 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J8I94_RS05730
• Protein ID: WP_000126304.1
• Coordinates: 1183135..1183554 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8I94_RS05695 (1177971)	1177971..1179680	+	1710	WP_001288123.1	formate hydrogenlyase subunit HycE	-
J8I94_RS05700 (1179690)	1179690..1180232	+	543	WP_000493800.1	formate hydrogenlyase subunit HycF	-
J8I94_RS05705 (1180232)	1180232..1180999	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
J8I94_RS05710 (1180996)	1180996..1181406	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
J8I94_RS05715 (1181399)	1181399..1181866	+	468	WP_000132955.1	hydrogenase maturation peptidase HycI	-
J8I94_RS05720 (1181909)	1181909..1182664	+	756	WP_001314074.1	hypothetical protein	-
J8I94_RS05725 (1182827)	1182827..1183138	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
J8I94_RS05730 (1183135)	1183135..1183554	+	420	WP_000126304.1	helix-turn-helix domain-containing protein	Antitoxin
J8I94_RS05735 (1183646)	1183646..1184074	-	429	WP_000536066.1	DUF4259 domain-containing protein	-
J8I94_RS05740 (1184459)	1184459..1184986	+	528	WP_001078777.1	electron transport protein HydN	-
J8I94_RS05745 (1185139)	1185139..1187391	+	2253	WP_001535518.1	carbamoyltransferase HypF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T198237 WP_000547563.1 NZ_CP072686:1182827-1183138 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T198237 NZ_CP096843:2832196-2832573 [Mycobacterium tuberculosis variant bovis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15474.50 Da
• Isoelectric Point: 4.4596

>AT198237 WP_000126304.1 NZ_CP072686:1183135-1183554 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT198237 NZ_CP096843:2831942-2832199 [Mycobacterium tuberculosis variant bovis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22