Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 308577..309163 Replicon chromosome
Accession NZ_CP072653
Organism Klebsiella pneumoniae strain KPN234

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag J8J76_RS01455 Protein ID WP_002920800.1
Coordinates 308795..309163 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag J8J76_RS01450 Protein ID WP_004174006.1
Coordinates 308577..308798 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8J76_RS01430 (J8J76_01430) 304734..305660 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
J8J76_RS01435 (J8J76_01435) 305657..306934 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
J8J76_RS01440 (J8J76_01440) 306931..307698 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
J8J76_RS01445 (J8J76_01445) 307700..308413 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
J8J76_RS01450 (J8J76_01450) 308577..308798 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
J8J76_RS01455 (J8J76_01455) 308795..309163 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
J8J76_RS01460 (J8J76_01460) 309436..310752 + 1317 WP_019704662.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
J8J76_RS01465 (J8J76_01465) 310859..311746 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
J8J76_RS01470 (J8J76_01470) 311743..312588 + 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
J8J76_RS01475 (J8J76_01475) 312590..313660 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 305657..314397 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T198203 WP_002920800.1 NZ_CP072653:308795-309163 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T198203 NZ_CP096843:704546-704947 [Mycobacterium tuberculosis variant bovis]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT198203 WP_004174006.1 NZ_CP072653:308577-308798 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT198203 NZ_CP096843:704304-704549 [Mycobacterium tuberculosis variant bovis]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References