Detailed information of TA system TA198159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1135958..1136597
• Replicon: chromosome
• Accession: NZ_CP072610
• Organism: Pseudomonas wenzhouensis strain A20

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: -
• Locus tag: J7655_RS05170
• Protein ID: WP_230926864.1
• Coordinates: 1135958..1136272 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J7655_RS05175
• Protein ID: WP_230926865.1
• Coordinates: 1136265..1136597 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7655_RS05155 (J7655_05145)	1132259..1133188	+	930	WP_230926861.1	D-2-hydroxyacid dehydrogenase family protein	-
J7655_RS05160 (J7655_05150)	1133362..1134306	+	945	WP_230926862.1	LysR family transcriptional regulator	-
J7655_RS05165 (J7655_05155)	1134352..1135548	-	1197	WP_230926863.1	MFS transporter	-
J7655_RS05170 (J7655_05160)	1135958..1136272	+	315	WP_230926864.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J7655_RS05175 (J7655_05165)	1136265..1136597	+	333	WP_230926865.1	XRE family transcriptional regulator	Antitoxin
J7655_RS05180 (J7655_05170)	1137155..1138240	-	1086	WP_011913346.1	IS5-like element ISPst12 family transposase	-
J7655_RS05185 (J7655_05175)	1138649..1138885	+	237	Protein_1021	transposase	-
J7655_RS05190 (J7655_05180)	1139401..1140144	-	744	WP_230926866.1	cache domain-containing protein	-
J7655_RS05195 (J7655_05185)	1140249..1141004	-	756	WP_230926867.1	GntR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (2-89)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-73)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11909.13 Da
• Isoelectric Point: 5.1828

>T198159 WP_230926864.1 NZ_CP072610:1135958-1136272 [Pseudomonas wenzhouensis]
MSAEEQESVAVSVQLLEERGPSLGFPHSSGINGSRHSHMRELRTQHEGRSYRTLYAFDPRRSAILLIDGDKTGDDRWYDI
NVPLSDRLYDEHLEQLRKEGLIDG

Nucleotide

• Length: 315 bp

>T198159 NZ_CP096842:1240703-1241077 [Mycobacterium tuberculosis variant bovis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12254.24 Da
• Isoelectric Point: 7.3202

>AT198159 WP_230926865.1 NZ_CP072610:1136265-1136597 [Pseudomonas wenzhouensis]
MAKKFAELRAKMAPEAQARVEAKAQELLAEMPLHELRQARGLSQKVLAEVLHVQQPSIAKMEKRTDIYISTLRSHIEAMG
GQLDVIARFPDGSVKISNFSDIESDLLLQA

Nucleotide

• Length: 333 bp

>AT198159 NZ_CP096842:1240509-1240706 [Mycobacterium tuberculosis variant bovis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA