Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yefM-yoeB (relBE)/Txe-RelB |
| Location | 229557..230092 | Replicon | plasmid pVB976_p1 |
| Accession | NZ_CP072587 | ||
| Organism | Enterococcus faecium strain VB976 | ||
Toxin (Protein)
| Gene name | yoeB | Uniprot ID | R2PQC0 |
| Locus tag | J7300_RS14765 | Protein ID | WP_002314366.1 |
| Coordinates | 229557..229829 (-) | Length | 91 a.a. |
Antitoxin (Protein)
| Gene name | yefM | Uniprot ID | Q3Y220 |
| Locus tag | J7300_RS14770 | Protein ID | WP_002297422.1 |
| Coordinates | 229829..230092 (-) | Length | 88 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| J7300_RS14725 (J7300_14750) | 224664..225113 | - | 450 | WP_010729386.1 | TlpA disulfide reductase family protein | - |
| J7300_RS14730 (J7300_14755) | 225269..225811 | - | 543 | WP_002298088.1 | hypothetical protein | - |
| J7300_RS14735 (J7300_14760) | 226086..226729 | + | 644 | Protein_250 | transposase | - |
| J7300_RS14740 (J7300_14765) | 226805..227485 | + | 681 | WP_002331383.1 | IS6-like element IS1216 family transposase | - |
| J7300_RS14745 (J7300_14770) | 227544..227732 | - | 189 | Protein_252 | hypothetical protein | - |
| J7300_RS14750 (J7300_14775) | 227733..228590 | - | 858 | WP_002292680.1 | ParA family protein | - |
| J7300_RS14755 (J7300_14780) | 228875..229161 | - | 287 | Protein_254 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| J7300_RS14760 (J7300_14785) | 229151..229480 | - | 330 | WP_002292678.1 | AbrB family transcriptional regulator | - |
| J7300_RS14765 (J7300_14790) | 229557..229829 | - | 273 | WP_002314366.1 | Txe/YoeB family addiction module toxin | Toxin |
| J7300_RS14770 (J7300_14795) | 229829..230092 | - | 264 | WP_002297422.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| J7300_RS14775 (J7300_14800) | 230326..231234 | + | 909 | Protein_258 | IS982-like element ISEfm1 family transposase | - |
| J7300_RS14780 (J7300_14805) | 231237..232412 | - | 1176 | WP_002336205.1 | MFS transporter | - |
| J7300_RS14785 (J7300_14810) | 232415..234664 | - | 2250 | WP_002314387.1 | alpha-galactosidase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | aac(6')-aph(2'') | - | 1..242945 | 242945 | |
| - | inside | IScluster/Tn | - | - | 217995..240693 | 22698 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 91 a.a. Molecular weight: 10780.24 Da Isoelectric Point: 9.9724
>T198135 WP_002314366.1 NZ_CP072587:c229829-229557 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ
Download Length: 273 bp
>T198135 NZ_CP096841:c3659793-3659365 [Mycobacterium tuberculosis variant bovis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG
Antitoxin
Download Length: 88 a.a. Molecular weight: 9959.22 Da Isoelectric Point: 4.5370
>AT198135 WP_002297422.1 NZ_CP072587:c230092-229829 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL
Download Length: 264 bp
>AT198135 NZ_CP096841:c3660039-3659797 [Mycobacterium tuberculosis variant bovis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829F704 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829F006 |