Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 229557..230092 Replicon plasmid pVB976_p1
Accession NZ_CP072587
Organism Enterococcus faecium strain VB976

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag J7300_RS14765 Protein ID WP_002314366.1
Coordinates 229557..229829 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag J7300_RS14770 Protein ID WP_002297422.1
Coordinates 229829..230092 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7300_RS14725 (J7300_14750) 224664..225113 - 450 WP_010729386.1 TlpA disulfide reductase family protein -
J7300_RS14730 (J7300_14755) 225269..225811 - 543 WP_002298088.1 hypothetical protein -
J7300_RS14735 (J7300_14760) 226086..226729 + 644 Protein_250 transposase -
J7300_RS14740 (J7300_14765) 226805..227485 + 681 WP_002331383.1 IS6-like element IS1216 family transposase -
J7300_RS14745 (J7300_14770) 227544..227732 - 189 Protein_252 hypothetical protein -
J7300_RS14750 (J7300_14775) 227733..228590 - 858 WP_002292680.1 ParA family protein -
J7300_RS14755 (J7300_14780) 228875..229161 - 287 Protein_254 type II toxin-antitoxin system RelE/ParE family toxin -
J7300_RS14760 (J7300_14785) 229151..229480 - 330 WP_002292678.1 AbrB family transcriptional regulator -
J7300_RS14765 (J7300_14790) 229557..229829 - 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
J7300_RS14770 (J7300_14795) 229829..230092 - 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
J7300_RS14775 (J7300_14800) 230326..231234 + 909 Protein_258 IS982-like element ISEfm1 family transposase -
J7300_RS14780 (J7300_14805) 231237..232412 - 1176 WP_002336205.1 MFS transporter -
J7300_RS14785 (J7300_14810) 232415..234664 - 2250 WP_002314387.1 alpha-galactosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..242945 242945
- inside IScluster/Tn - - 217995..240693 22698


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T198135 WP_002314366.1 NZ_CP072587:c229829-229557 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T198135 NZ_CP096841:c3659793-3659365 [Mycobacterium tuberculosis variant bovis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT198135 WP_002297422.1 NZ_CP072587:c230092-229829 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT198135 NZ_CP096841:c3660039-3659797 [Mycobacterium tuberculosis variant bovis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References