Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 220568..221103 Replicon plasmid pVB12993_p1
Accession NZ_CP072580
Organism Enterococcus faecium strain VB12993

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag J7299_RS14975 Protein ID WP_002314366.1
Coordinates 220568..220840 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag J7299_RS14980 Protein ID WP_002297422.1
Coordinates 220840..221103 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7299_RS14935 (J7299_14935) 215674..216123 - 450 WP_010729386.1 TlpA disulfide reductase family protein -
J7299_RS14940 (J7299_14940) 216279..216821 - 543 WP_002298088.1 hypothetical protein -
J7299_RS14945 (J7299_14945) 217096..217739 + 644 Protein_237 transposase -
J7299_RS14950 (J7299_14950) 217815..218495 + 681 WP_002331383.1 IS6-like element IS1216 family transposase -
J7299_RS14955 (J7299_14955) 218554..218742 - 189 Protein_239 hypothetical protein -
J7299_RS14960 (J7299_14960) 218743..219600 - 858 WP_002292680.1 ParA family protein -
J7299_RS14965 (J7299_14965) 219886..220172 - 287 Protein_241 type II toxin-antitoxin system RelE/ParE family toxin -
J7299_RS14970 (J7299_14970) 220162..220491 - 330 WP_002292678.1 AbrB family transcriptional regulator -
J7299_RS14975 (J7299_14975) 220568..220840 - 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
J7299_RS14980 (J7299_14980) 220840..221103 - 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
J7299_RS14985 (J7299_14985) 221337..222245 + 909 Protein_245 IS982-like element ISEfm1 family transposase -
J7299_RS14990 (J7299_14990) 222248..223423 - 1176 WP_002336205.1 MFS transporter -
J7299_RS14995 (J7299_14995) 223426..225675 - 2250 WP_002314387.1 alpha-galactosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..235463 235463
- inside IScluster/Tn - - 209005..233211 24206


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T198128 WP_002314366.1 NZ_CP072580:c220840-220568 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T198128 NZ_CP096841:c3026490-3026095 [Mycobacterium tuberculosis variant bovis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT198128 WP_002297422.1 NZ_CP072580:c221103-220840 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT198128 NZ_CP096841:c3026069-3025803 [Mycobacterium tuberculosis variant bovis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References