Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3702875..3703416 Replicon chromosome
Accession NZ_CP072564
Organism Escherichia coli strain WG5::PA2

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag J8F87_RS17890 Protein ID WP_000615979.1
Coordinates 3703138..3703416 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag J8F87_RS17885 Protein ID WP_000729704.1
Coordinates 3702875..3703135 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8F87_RS17865 (3698945) 3698945..3699730 - 786 WP_000207555.1 putative lateral flagellar export/assembly protein LafU -
J8F87_RS17870 (3699702) 3699702..3701424 + 1723 Protein_3510 flagellar biosynthesis protein FlhA -
J8F87_RS17875 (3701503) 3701503..3701859 + 357 WP_047402977.1 type II toxin-antitoxin system HicB family antitoxin -
J8F87_RS17880 (3701916) 3701916..3702689 - 774 WP_122990173.1 C40 family peptidase -
J8F87_RS17885 (3702875) 3702875..3703135 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
J8F87_RS17890 (3703138) 3703138..3703416 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
J8F87_RS17895 (3703572) 3703572..3704312 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
J8F87_RS17900 (3704283) 3704283..3705050 - 768 WP_000333380.1 class II glutamine amidotransferase -
J8F87_RS17905 (3705259) 3705259..3705837 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T198069 WP_000615979.1 NZ_CP072564:3703138-3703416 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T198069 NZ_CP096840:2899054-2899494 [Mycobacterium tuberculosis variant bovis]
ATGCTGCTCTGTGACACCAACATCTGGCTGGCGTTGGCGCTTTCCGGACACGTGCACCACAGGGCCTCGCGCGCATGGCT
AGACACCATCAACGCGCCCGGAGTCATCCACTTTTGCCGCGCAACCCAACAGTCGCTCCTTCGGCTGTTGACGAATCGGA
CGGTGCTGGGCGCGTATGGCAGCCCACCACTGACCAACCGCGAAGCGTGGGCGGCCTATGCCGCGTTCCTGGATGACGAC
CGCATCGTGCTGGCCGGCGCCGAACCTGATGGTTTGGAGGCCCAGTGGAGAGCCTTCGCCGTTCGCCAGTCGCCGGCGCC
CAAGGTTTGGATGGATGCCTACCTAGCTGCTTTCGCACTTACCGGTGGATTCGAGTTGGTGACGACTGACACCGCCTTCA
CCCAGTACGGCGGAATCGAGCTGCGGCTCCTGGCCAAGTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT198069 WP_000729704.1 NZ_CP072564:3702875-3703135 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT198069 NZ_CP096840:2898780-2899067 [Mycobacterium tuberculosis variant bovis]
ATGAAGACCACGCTCGACCTGCCTGATGAACTGATGCGCGCTATCAAGGTCCGCGCGGCGCAGCAGGGCCGCAAGATGAA
AGATGTCGTGACCGAACTGCTCAGATCCGGTCTGTCCCAGACGCACAGCGGGGCTCCAATCCCAACGCCGCGGCGCGTGC
AGCTTCCCCTGGTGCATTGCGGTGGCGCGGCTACCCGCGAACAAGAAATGACGCCGGAGCGTGTTGCCGCGGCCTTGCTC
GACCAGGAGGCCCAGTGGTGGTCCGGACACGACGATGCTGCTCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References