Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 73110..73837 | Replicon | plasmid pKP2648-Vir |
| Accession | NZ_CP072558 | ||
| Organism | Klebsiella pneumoniae strain KP2648 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A7W3D9W1 |
| Locus tag | J7339_RS28520 | Protein ID | WP_011251285.1 |
| Coordinates | 73110..73421 (+) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | J7339_RS28525 | Protein ID | WP_011251286.1 |
| Coordinates | 73418..73837 (+) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| J7339_RS28480 (J7339_28385) | 68121..68615 | + | 495 | WP_011251274.1 | hypothetical protein | - |
| J7339_RS28485 (J7339_28390) | 68621..69061 | + | 441 | WP_011251275.1 | hypothetical protein | - |
| J7339_RS28490 (J7339_28395) | 69486..70442 | - | 957 | WP_011251280.1 | DsbA family protein | - |
| J7339_RS28495 (J7339_28400) | 70502..70843 | - | 342 | WP_011251281.1 | hypothetical protein | - |
| J7339_RS28500 (J7339_28405) | 70857..71168 | - | 312 | WP_011251282.1 | hypothetical protein | - |
| J7339_RS28505 (J7339_28410) | 71185..71634 | - | 450 | WP_011251283.1 | thioredoxin fold domain-containing protein | - |
| J7339_RS28515 (J7339_28420) | 72468..72905 | + | 438 | Protein_86 | DDE-type integrase/transposase/recombinase | - |
| J7339_RS28520 (J7339_28425) | 73110..73421 | + | 312 | WP_011251285.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| J7339_RS28525 (J7339_28430) | 73418..73837 | + | 420 | WP_011251286.1 | helix-turn-helix domain-containing protein | Antitoxin |
| J7339_RS28530 (J7339_28435) | 73984..74952 | + | 969 | WP_074428168.1 | IS5 family transposase | - |
| J7339_RS28535 (J7339_28440) | 75024..75389 | - | 366 | WP_048333448.1 | hypothetical protein | - |
| J7339_RS28540 (J7339_28445) | 75403..76191 | - | 789 | WP_040217257.1 | hypothetical protein | - |
| J7339_RS28545 (J7339_28450) | 76212..76832 | - | 621 | WP_223175004.1 | conjugative transfer protein MobI(A/C) | - |
| J7339_RS28550 (J7339_28455) | 77251..77886 | + | 636 | WP_223171879.1 | hypothetical protein | - |
| J7339_RS28555 (J7339_28460) | 78199..78669 | + | 471 | WP_048333570.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | iucD / iucC / iucB / iucA / rmpA / iroN / rmpA2 / iutA | 1..216902 | 216902 | |
| - | inside | IScluster/Tn | - | - | 66201..90508 | 24307 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12346.12 Da Isoelectric Point: 9.7248
>T198042 WP_011251285.1 NZ_CP072558:73110-73421 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
Download Length: 312 bp
>T198042 NZ_CP096840:1012489-1012923 [Mycobacterium tuberculosis variant bovis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA
Antitoxin
Download Length: 140 a.a. Molecular weight: 15377.46 Da Isoelectric Point: 4.4420
>AT198042 WP_011251286.1 NZ_CP072558:73418-73837 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
Download Length: 420 bp
>AT198042 NZ_CP096840:1012304-1012483 [Mycobacterium tuberculosis variant bovis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCAGGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCAGGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|