Detailed information of TA system TA198037

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 71530..71783
• Replicon: plasmid pKP2648-KPC
• Accession: NZ_CP072557
• Organism: Klebsiella pneumoniae strain KP2648

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: J7339_RS27830
• Protein ID: WP_001312851.1
• Coordinates: 71530..71679 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	71724..71783 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7339_RS27795 (66889)	66889..67304	-	416	Protein_73	IS1-like element IS1B family transposase	-
J7339_RS27800 (67553)	67553..67954	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
J7339_RS27805 (67887)	67887..68144	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
J7339_RS27810 (68237)	68237..68890	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
J7339_RS27815 (69829)	69829..70686	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
J7339_RS29305 (70679)	70679..70753	-	75	WP_001375168.1	RepA leader peptide Tap	-
J7339_RS27825 (70998)	70998..71246	-	249	WP_000083837.1	replication regulatory protein RepA	-
J7339_RS27830 (71530)	71530..71679	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (71724)	71724..71783	+	60	NuclAT_0	-	Antitoxin
- (71724)	71724..71783	+	60	NuclAT_0	-	Antitoxin
- (71724)	71724..71783	+	60	NuclAT_0	-	Antitoxin
- (71724)	71724..71783	+	60	NuclAT_0	-	Antitoxin
J7339_RS27835 (71984)	71984..72316	-	333	WP_152916585.1	hypothetical protein	-
J7339_RS27840 (72378)	72378..72977	-	600	WP_032083981.1	PIN domain-containing protein	-
J7339_RS27845 (73363)	73363..73563	-	201	WP_015059022.1	hypothetical protein	-
J7339_RS27850 (73695)	73695..74255	-	561	WP_000139328.1	fertility inhibition protein FinO	-
J7339_RS27855 (74310)	74310..75056	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
J7339_RS27860 (75076)	75076..75276	-	201	WP_072354025.1	hypothetical protein	-
J7339_RS27865 (75301)	75301..76005	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
J7339_RS27870 (76060)	76060..76425	-	366	Protein_88	type IV conjugative transfer system protein TraE	-
J7339_RS27875 (76445)	76445..76750	-	306	WP_004144424.1	type IV conjugative transfer system protein TraL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaSHV-187 / blaCTX-M-65	-	1..102746	102746
	-	flank	IS/Tn	-	-	75301..76005	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T198037 WP_001312851.1 NZ_CP072557:c71679-71530 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T198037 NZ_CP096840:c752114-751731 [Mycobacterium tuberculosis variant bovis]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin

Nucleotide

• Length: 60 bp

>AT198037 NZ_CP072557:71724-71783 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin