Detailed information of TA system TA198016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3858..4509
• Replicon: plasmid pH7-68
• Accession: NZ_CP072552
• Organism: Acinetobacter lwoffii strain H7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: J7649_RS16080
• Protein ID: WP_092818271.1
• Coordinates: 4153..4509 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J7649_RS16075
• Protein ID: WP_001140620.1
• Coordinates: 3858..4160 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7649_RS16055 (J7649_16055)	51..1385	-	1335	WP_219310253.1	ISNCY family transposase	-
J7649_RS16060 (J7649_16060)	1741..2562	+	822	WP_219310255.1	AraC family transcriptional regulator	-
J7649_RS16065 (J7649_16065)	2584..3189	-	606	WP_219310257.1	LysE family transporter	-
J7649_RS16070 (J7649_16070)	3392..3798	+	407	Protein_3	DUF2000 domain-containing protein	-
J7649_RS16075 (J7649_16075)	3858..4160	-	303	WP_001140620.1	XRE family transcriptional regulator	Antitoxin
J7649_RS16080 (J7649_16080)	4153..4509	-	357	WP_092818271.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J7649_RS16085 (J7649_16085)	5302..5631	+	330	WP_016658833.1	four-helix bundle copper-binding protein	-
J7649_RS16090 (J7649_16090)	5760..6052	-	293	Protein_7	type II toxin-antitoxin system RelE/ParE family toxin	-
J7649_RS16095 (J7649_16095)	6042..6287	-	246	WP_000246755.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
J7649_RS16100 (J7649_16100)	6397..7722	-	1326	WP_081400761.1	ISNCY family transposase	-
J7649_RS16105 (J7649_16105)	7954..8898	-	945	WP_104510421.1	copper resistance protein B	-
J7649_RS16110 (J7649_16110)	8885..9223	-	339	WP_004897649.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..68402	68402
	-	flank	IS/Tn	-	-	51..1373	1322

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13501.46 Da
• Isoelectric Point: 6.4623

>T198016 WP_092818271.1 NZ_CP072552:c4509-4153 [Acinetobacter lwoffii]
MWTVITTDLFNQWLEQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIADKGGKKRFYTEMLAIADQQYELHLSTLGEQSNG

Nucleotide

• Length: 357 bp

>T198016 NZ_CP096839:c3097011-3096127 [Mycobacterium tuberculosis variant bovis]
GTGGCCGGTCTGACCCGTGCGCTCGTTGCGCGTCACGCTCTTGGTCGGGCCGAGGCATACGACGCCGCTCTGCTCGACGT
CGCCCAGGACCACCTGCTGTACCTGCTGTCGCAGACCGTCCAGTTCGGCGATAACCGCCTGGTGTTCAAAGGCGGTACCA
GCTTACGGAAGTGCCGGCTGGGCAACGTCGGGCGGTTCTCCACTGATCTGGACTTCAGCGCGCCCGACGATGAGGTCGTG
CTGGAGGTGTGCGAGCTGATCGACGGCGCCCGTGTCGGCGGCTTCGAATTCGGCGTGCAGAGCACACGAGGCGACGGCCG
GCATTGGCAGTTGCGGGTGCGGCACACGGAATTGGGCGAGCCCCGGATCGTCGCCAGCGTCGAGTTCGCACGGCGGCCGT
TGGCTTTGCCGTCTGAGCTGCTCGCATTCATCCAGTTGCCGATCCATAAGGCTTACGGTTTTGGGCTGCCGACACTGCCG
GTCGTGGCAGAAGCGGAAGCCTGCGCAGAGAAACTCGCGCGGTACCGCCGCGTTGCGCTGGCACGGGACCTGTACGACCT
GAACCACTTCGCCTCGCGAACGATTGACGAACCGCTCGTGCGGCGGCTGTGGGTGCTCAAGGTGTGGGGTGATGTCGTCG
ATGACCGGCGCGGCACCCGGCCACTACGCGTCGAAGACGTCCTCGCCGCCCGCAGCGAGCACGACTTCCAGCCCGACTCG
ATCGGCGTGCTGACCCGTCCTGTCGCTATGGCTGCCTGGGAAGCTCGCGTTCGGAAGCGATTTGCGTTCCTCACTGACCT
CGACGCCGACGAGCAGCGGTGGGCCGCCTGCGACGAACGGCACCGCCGCGAAGTGGAGAACGCGCTGGCGGTGCTGCGGT
CCTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11227.02 Da
• Isoelectric Point: 6.4759

>AT198016 WP_001140620.1 NZ_CP072552:c4160-3858 [Acinetobacter lwoffii]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT198016 NZ_CP096839:c3097901-3097014 [Mycobacterium tuberculosis variant bovis]
GTGGTGAGCCCAGCCGGCGCCGATCGGCGCATCCCCACGTGGGCGTCACGGGTGGTGTCCGGCCTCGCTCGCGATCGGCC
CGTAGTCGTCACGAAAGAGGATCTGACGCAACGGCTTACAGAGGCTGGGTGTGGACGCGACCCCGACTCCGCCATCCGCG
AACTGCGCCGCATTGGGTGGCTGGTCCAACTGCCGGTGAAAGGCACCTGGGCATTCATTCCCCCCGGCGAGGCCGCCATC
TCGGACCCGTATCTACCGCTGCGCTCGTGGCTGGCCCGTGACCAGAACGCGGGCTTCATGCTGGCCGGTGCATCCGCAGC
GTGGCACCTCGGATACCTGGACCGCCAACCCGACGGCCGCATCCCGATCTGGCTGCCGCCGGCCAAAAGGCTGCCCGACG
GCCTAGCATCGTATGTGTCCGTCGTGCGCATCCCCTGGAACGCGGCGGACACCGCACTGCTGGCTCCGCGTCCCGCGTTG
CTGGTCCGGCGGCGGCTCGACCTCGTCGCGTGGGCGACCGGGTTACCGGCGCTAGGACCAGAAGCATTACTTGTGCAAAT
CGCCACGCGCCCGGCCTCGTTCGGGCCGTGGGCCGACCTTGTCCCCCACCTTGACGACCTCGTCGCCGACTGCAGTGACG
AGCGTCTGGAGCGTCTACTGTCGGGCCGGCCGACATCGGCCTGGCAGCGTGCAAGCTACCTGCTGGACTCGGGCGGTGAA
CCGGCACGAGGCCAGGCGCTGCTGGCCAAGCGGCACACCGAAGTGATGCCGGTGACTCGATTCACCACCGCGCACAGCCG
CGACCGTGGCGAGAGTGTCTGGGCTCCCGAGTATCAGCTTGTCGACGAGCTCGTCGTACCGCTGCTGCGCGTGATCGGCA
AGGCGTGA