Detailed information of TA system TA198015

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 24242..24893
• Replicon: plasmid pH7-108
• Accession: NZ_CP072551
• Organism: Acinetobacter lwoffii strain H7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: J7649_RS15620
• Protein ID: WP_103857265.1
• Coordinates: 24537..24893 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J7649_RS15615
• Protein ID: WP_001140620.1
• Coordinates: 24242..24544 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7649_RS15580 (J7649_15580)	19247..19570	-	324	WP_219310211.1	hypothetical protein	-
J7649_RS15585 (J7649_15585)	19847..20218	+	372	WP_219310212.1	hypothetical protein	-
J7649_RS15590 (J7649_15590)	20201..20794	-	594	Protein_19	DNA-directed DNA polymerase	-
J7649_RS15595 (J7649_15595)	21415..22119	-	705	WP_001067784.1	IS6-like element IS1006 family transposase	-
J7649_RS15600 (J7649_15600)	22183..23463	-	1281	WP_005093338.1	cation transporter	-
J7649_RS15605 (J7649_15605)	23553..23945	+	393	WP_000550240.1	Cd(II)/Pb(II)-responsive transcriptional regulator	-
J7649_RS15610 (J7649_15610)	24039..24200	+	162	Protein_23	IS6 family transposase	-
J7649_RS15615 (J7649_15615)	24242..24544	-	303	WP_001140620.1	XRE family transcriptional regulator	Antitoxin
J7649_RS15620 (J7649_15620)	24537..24893	-	357	WP_103857265.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J7649_RS15625 (J7649_15625)	25415..26386	-	972	WP_005176780.1	helix-turn-helix domain-containing protein	-
J7649_RS15630 (J7649_15630)	26519..27403	+	885	WP_178929016.1	MBL fold metallo-hydrolase	-
J7649_RS15635 (J7649_15635)	27488..28030	+	543	WP_004637127.1	cysteine hydrolase family protein	-
J7649_RS15640 (J7649_15640)	28120..28425	-	306	WP_005020909.1	helix-turn-helix domain-containing protein	-
J7649_RS16840	28409..28612	-	204	Protein_30	transcriptional regulator	-
J7649_RS15645 (J7649_15645)	28664..29368	-	705	WP_001067784.1	IS6-like element IS1006 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..108848	108848
	-	inside	IScluster/Tn	-	-	21415..29368	7953

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13558.56 Da
• Isoelectric Point: 7.1647

>T198015 WP_103857265.1 NZ_CP072551:c24893-24537 [Acinetobacter lwoffii]
MWTVITTDLFNQWLEQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Nucleotide

• Length: 357 bp

>T198015 NZ_CP096839:c3072756-3072400 [Mycobacterium tuberculosis variant bovis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11227.02 Da
• Isoelectric Point: 6.4759

>AT198015 WP_001140620.1 NZ_CP072551:c24544-24242 [Acinetobacter lwoffii]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT198015 NZ_CP096839:c3072970-3072740 [Mycobacterium tuberculosis variant bovis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA