Detailed information of TA system TA198012

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 237066..237651
• Replicon: plasmid pH7-250
• Accession: NZ_CP072550
• Organism: Acinetobacter lwoffii strain H7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: J7649_RS15405
• Protein ID: WP_000897307.1
• Coordinates: 237331..237651 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J7649_RS15400
• Protein ID: WP_000369781.1
• Coordinates: 237066..237338 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7649_RS15385 (J7649_15385)	232781..233920	-	1140	Protein_216	ISNCY family transposase	-
J7649_RS15390 (J7649_15390)	234158..235042	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
J7649_RS15395 (J7649_15395)	235098..236573	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
J7649_RS15400 (J7649_15400)	237066..237338	-	273	WP_000369781.1	NadS family protein	Antitoxin
J7649_RS15405 (J7649_15405)	237331..237651	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J7649_RS15410 (J7649_15410)	237775..239100	-	1326	WP_001280601.1	ISNCY family transposase	-
J7649_RS15415 (J7649_15415)	239278..239697	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
J7649_RS15420 (J7649_15420)	239777..240058	-	282	WP_000985609.1	putative addiction module antidote protein	-
J7649_RS15425 (J7649_15425)	240059..240361	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
J7649_RS15430 (J7649_15430)	240481..241806	-	1326	WP_001280601.1	ISNCY family transposase	-
J7649_RS15435 (J7649_15435)	241997..242527	+	531	WP_000172759.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-VIa / sul1 / blaPER-1 / qacE / ARR-3 / floR / mph(E) / msr(E)	-	1..250175	250175
	-	inside	IScluster/Tn	floR / mph(E) / msr(E)	-	229697..246245	16548

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T198012 WP_000897307.1 NZ_CP072550:c237651-237331 [Acinetobacter lwoffii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T198012 NZ_CP096839:2903461-2903901 [Mycobacterium tuberculosis variant bovis]
ATGCTGCTCTGTGACACCAACATCTGGCTGGCGTTGGCGCTTTCCGGACACGTGCACCACAGGGCCTCGCGCGCATGGCT
AGACACCATCAACGCGCCCGGAGTCATCCACTTTTGCCGCGCAACCCAACAGTCGCTCCTTCGGCTGTTGACGAATCGGA
CGGTGCTGGGCGCGTATGGCAGCCCACCACTGACCAACCGCGAAGCGTGGGCGGCCTATGCCGCGTTCCTGGATGACGAC
CGCATCGTGCTGGCCGGCGCCGAACCTGATGGTTTGGAGGCCCAGTGGAGAGCCTTCGCCGTTCGCCAGTCGCCGGCGCC
CAAGGTTTGGATGGATGCCTACCTAGCTGCTTTCGCACTTACCGGTGGATTCGAGTTGGTGACGACTGACACCGCCTTCA
CCCAGTACGGCGGAATCGAGCTGCGGCTCCTGGCCAAGTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT198012 WP_000369781.1 NZ_CP072550:c237338-237066 [Acinetobacter lwoffii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT198012 NZ_CP096839:2903187-2903474 [Mycobacterium tuberculosis variant bovis]
ATGAAGACCACGCTCGACCTGCCTGATGAACTGATGCGCGCTATCAAGGTCCGCGCGGCGCAGCAGGGCCGCAAGATGAA
AGATGTCGTGACCGAACTGCTCAGATCCGGTCTGTCCCAGACGCACAGCGGGGCTCCAATCCCAACGCCGCGGCGCGTGC
AGCTTCCCCTGGTGCATTGCGGTGGCGCGGCTACCCGCGAACAAGAAATGACGCCGGAGCGTGTTGCCGCGGCCTTGCTC
GACCAGGAGGCCCAGTGGTGGTCCGGACACGACGATGCTGCTCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3